Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- SM7026P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM7026P, RRID:AB_980461
- Product name
- anti DNMT1
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide corresponding to amino acids 637-651 of Human Dnmt1 (NP_001370).
- Reactivity
- Human, Mouse, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 60B1220.1
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Dnmt1 in 2102EP (Human embryonic carcinoma). 0.5 µg/ml of SM7026P was used for testing the Human cell line and 0.1 µg/ml was used for testing the recombinant protein and the Mouse cell lines in A) absence and B) presence of immunizing peptide, recombinant Human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (Mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (Mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using SM7026P. Goat anti-Mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- SM7026P Dnmt1 antibody staining of Formalin-Fixed, Paraffin-Embedded medullar kidney tissue sections at 2 µg/ml.