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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- LS-C801228 - Provider product page
- Provider
- LSBio
- Product name
- FANCD2 Antibody (phospho-Ser222) LS-C801228
- Antibody type
- Polyclonal
- Description
- Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of Phospho-FANCD2 (Ser222) antibody expression in CalyculinA treated HT29 cells lysates. The lane on the right is treated with the antigen-specific peptide.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of FANCD2 phosphorylation expression in CalyculinA treated HT29 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- 1:100 staining mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- 1:100 staining mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- 1/100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
