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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [6]
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- Product number
- PA5-92849 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KAO Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EPSPGTLPRK AGVFSDLSNQ ELKAVHSFLW SKKELRLQPS STTTMAKNTV FLIEMLLPKK YHVLRFLDKG ERHPVREARA VIFFGDQEHP NVTEFAVGPL PGPCYMRALS PRPGYQSSWA SRPISTAEYA LLYHTLQEAT KPLHQFFLNT TGFSFQDCHD RCLAFTDVAP RGVASGQRRS WLIIQRYVEG YFLHPTGLEL LVDHGSTDAG HWAVEQVWYN GKFYGSPEEL ARKYADGEVD VVVLEDPLPG GKGHDSTEEP PLFSSHKPRG DFPSPIHVSG P; Positive Samples: Mouse kidney, Mouse liver, Mouse heart, Rat kidney, Rat heart; Cellular Location: extracellular space
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.08 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KAO in extracts of various cell lines. Samples were incubated with KAO Polyclonal antibody (Product # PA5-92849) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of KAO was performed in HeLa cells using KAO Polyclonal Antibody (Product # PA5-92849) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of KAO was performed in NIH-3T3 cells using KAO Polyclonal Antibody (Product # PA5-92849) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of KAO was performed in PC-12 cells using KAO Polyclonal Antibody (Product # PA5-92849) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of KAO in HeLa cells. Samples were incubated with KAO Polyclonal antibody (Product # PA5-92849) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of KAO in NIH-3T3 cells. Samples were incubated with KAO Polyclonal antibody (Product # PA5-92849) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of KAO in PC-12 cells. Samples were incubated with KAO Polyclonal antibody (Product # PA5-92849) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
