692402

antibody from BioLegend

Targeting: TERF2 TRBF2, TRF2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

692402

Provider

BioLegend

Proper citation

BioLegend Cat#692402, RRID:AB_2632764

Product name

Purified anti-TRF2

Antibody type

Monoclonal

Description

Telomeric Repeat-binding Factor 2 (TRF2) is a component of the shelterin complex, which is a six-protein complex bound to chromosome termini. The shelterin complex is essential for protecting the integrity of natural chromosome ends. Within the shelterin complex, POT1 binds single-stranded telomeric overhang, while TRF1 and TRF2 bind duplex telomeric DNA and recruit TIN2, TPP1, POT1 and Rap1 to telomeres. Inhibition of TRF2 activates DNA repair systems specifically at telomeric loci, leading to cell cycle arrest, senescence or cell death. Over-expression of TRF2 was observed in a variety of human cancers, suggesting TRF2’s roles in tumorigenesis.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

T2JKM3

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total protein extracts (15 µg protein) from HeLa and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-TRF2 (clone T2JKM3) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.

Conjugate

Unconjugated