H00000324-M01

antibody from Abnova Corporation

Targeting: APC DP2, DP2.5, DP3, PPP1R46

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000324-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000324-M01, RRID:AB_463862

Product name

APC monoclonal antibody (M01), clone 3E2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant APC.

Antigen sequence

NPVPVSETNESSIVERTPFSSSSSSKHSSPSGTVA
ARVTPFNYNPSPRKSSADSTSARPSQIPTPVNNNT
KKRDSKTDSTESSGTQSPKRHSGSYLVTSV


Isotype

IgG

Antibody clone number

3E2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to APC on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between JUP and APC. HeLa cells were stained with anti-JUP rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)