Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-96213 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Protein APC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QSKENQVSAK GTWRKIKENE FSPTNSTSQT VSSGATNGAE SKTLIYQMAP AVSKTEDVWV RIEDCPINNP RSGRSPTGNT PPVIDSVSEK ANPNIKDSKD NQAKQNVGNG SVPMRTVGLE NRLNSFIQVD APDQKGTEIK PGQNNPVPVS ETNESSIVER T Positive Samples: HeLa; Cellular Location: Cell junction, Cell membrane, Cell projection, Cytoplasm, adherens junction, cytoskeleton, lamellipodium, ruffle membrane
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.27 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Protein APC in extracts of HeLa cells using Protein APC Polyclonal Antibody (Product # PA5-96213) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Protein APC was performed in HT-29 cells using Protein APC Polyclonal Antibody (Product # PA5-96213).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Protein APC in paraffin-embedded human lung using Protein APC Polyclonal Antibody (Product # PA5-96213) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Protein APC in paraffin-embedded human colon using Protein APC Polyclonal Antibody (Product # PA5-96213) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Protein APC in paraffin-embedded human breast cancer using Protein APC Polyclonal Antibody (Product # PA5-96213) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of Protein APC was performed in mouse colon tissue using Protein APC Polyclonal Antibody (Product # PA5-96213) at a dilution of 1:100. Blue: DAPI for nuclear staining.