Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations [0]
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- Product number
- 611402 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#611402, RRID:AB_2075011
- Product name
- Purified anti-APC8
- Antibody type
- Polyclonal
- Antigen
- Recombinant (partial), N-terminal
- Description
- APC8 (anaphase-promoting complex subunit 8) is a member of the E3 enzyme family. This protein contains TPR repeats and has a molecular weight of approximately 68 kD. The APC8 protein is located in the nucleus during interphase and the centrosome during metaphase/anaphase. This protein is required for mitotic anaphase transition. The APC8 protein functions with other members of the APC complex as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC8 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. APC8 is phosphorylated by cdk1-cyclinB and PLK-1. The Poly6114 antibody has been shown to be useful for Western blotting of the human and mouse APC8 protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20° C.
Submitted references Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F.
Accumulation of substrates of the anaphase-promoting complex (APC) during human cytomegalovirus infection is associated with the phosphorylation of Cdh1 and the dissociation and relocalization of APC subunits.
Gurden MD, Holland AJ, van Zon W, Tighe A, Vergnolle MA, Andres DA, Spielmann HP, Malumbres M, Wolthuis RM, Cleveland DW, Taylor SS
Journal of cell science 2010 Feb 1;123(Pt 3):321-30
Journal of cell science 2010 Feb 1;123(Pt 3):321-30
Accumulation of substrates of the anaphase-promoting complex (APC) during human cytomegalovirus infection is associated with the phosphorylation of Cdh1 and the dissociation and relocalization of APC subunits.
Tran K, Mahr JA, Choi J, Teodoro JG, Green MR, Spector DH
Journal of virology 2008 Jan;82(1):529-37
Journal of virology 2008 Jan;82(1):529-37
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