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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-30580 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Protein APC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 1.44 mg/mL
Submitted references Loss of Apc Cooperates with Activated Oncogenes to Induce Liver Tumor Formation in Mice.
Zhang Y, Liang B, Song X, Wang H, Evert M, Zhou Y, Calvisi DF, Tang L, Chen X
The American journal of pathology 2021 May;191(5):930-946
The American journal of pathology 2021 May;191(5):930-946
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Protein APC was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Protein APC Polyclonal Antibody (Product # PA5-30580) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of APC in methanol-fixed A431 cells using an APC polyclonal antibody (Product # PA5-30580) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Protein APC was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Protein APC Polyclonal Antibody (Product # PA5 30580) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Protein APC was performed in paraffin-embedded rat colon tissue using Protein APC Polyclonal Antibody (Product # PA5-30580) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Protein APC Polyclonal Antibody detects APC protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated). Sample: Paraffin-embedded human colon cancer. APC stained by Protein APC Polyclonal Antibody (Product # PA5-30580) diluted at 1:500. Antigen Retrieval: Citrate buffer, 20 min.
