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- Antibody Data
- Antigen structure
- References [1]
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- Product number
- MAB5889 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Klotho beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Klotho beta in direct ELISA and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q86Z14- Isotype
- IgG
- Antibody clone number
- 885935
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A systematic dissection of sequence elements determining β-Klotho and FGF interaction and signaling.
Shi SY, Lu YW, Richardson J, Min X, Weiszmann J, Richards WG, Wang Z, Zhang Z, Zhang J, Li Y
Scientific reports 2018 Jul 23;8(1):11045
Scientific reports 2018 Jul 23;8(1):11045
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Klotho beta by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB5889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Klotho beta at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Klotho beta by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Klotho beta at approximately 177/170 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB5889) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
