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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB2139 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat Laminin gamma 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and rat Laminin gamma 1.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- D18
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
Green TL, Hunter DD, Chan W, Merlie JP, Sanes JR
The Journal of biological chemistry 1992 Jan 25;267(3):2014-22
The Journal of biological chemistry 1992 Jan 25;267(3):2014-22
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Laminin gamma 1 in LS180 Human Cell Line. Laminin gamma 1 was detected in immersion fixed LS180 human colorectal adenocarcinoma cell line using Mouse Anti-Human/Rat Laminin gamma 1 Monoclonal Antibody (Catalog # MAB2139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
