PA5-13488

antibody from Invitrogen Antibodies

Targeting: VHL VHL1

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13488

Provider

Invitrogen Antibodies

Product name

VHL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

A highly potent PROTAC androgen receptor (AR) degrader ARD-61 effectively inhibits AR-positive breast cancer cell growth in vitro and tumor growth in vivo.

Zhao L, Han X, Lu J, McEachern D, Wang S
Neoplasia (New York, N.Y.) 2020 Oct;22(10):522-532

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HepG2 cells using a VHL polyclonal antibody (Product # PA5-13488) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 ARD-61 is a bona fide PROTAC degrader in human breast cancer cell lines. (A) Chemical structures of AR degrader ARD-61, AR antagonist ARi-16 and VHL ligand used for the design of ARD-61. (B) Cells were pretreated with AR antagonist ARi-16, VHL ligand (HXD079), neddylation activating E1 enzyme inhibitor MLN4924 and proteasome inhibitor MG132 for 2 h, followed by treatment with ARD-61 for another 6 h. AR protein levels were detected by Western blot and GAPDH was used as a loading control. (C) MDA-MB-543 cells were treated with ARD-61 or in combination with an AR agonist R1881 for 24 h. AR protein levels were detected by Western blot and GAPDH was used as a loading control. (D) MDA-MB-453 and MCF-7 cells were transfected with siVehicle (Control) or siVHL for 72 h and then treated with 100 nM of ARD-61 for another 24 h. AR protein levels were detected by Western blot and GAPDH was used as a loading control.