PAB28541

antibody from Abnova Corporation

Targeting: IRF4 LSIRF, MUM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28541

Provider

Abnova Corporation

Product name

IRF4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant IRF4.

Antigen sequence

SNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQ
LTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPP
LDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGP
ACENGCQVTGTFYACAPPE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with IRF4 polyclonal antibody (Cat # PAB28541) at 1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with IRF4 polyclonal antibody (Cat # PAB28541) at 1-4 ug/ml shows positivity in nucleus but not nucleoli and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human IRF4 polyclonal antibody (Cat # PAB28541) shows strong nuclear positivity in lymphoid cells outside reaction centra at 1:250 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)