646403

antibody from BioLegend

Targeting: IRF4 LSIRF, MUM1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

646403

Provider

BioLegend

Proper citation

BioLegend Cat#646403, RRID:AB_2563004

Product name

PE anti-IRF4

Antibody type

Monoclonal

Antigen

GST fusion protein containing C-terminal of murine IRF4

Description

The IRF family consists of at least nine members. IRF4 and IRF8 are highly homologous to each other and also redundant in function. IRF4 is critical for Th2 and Th17 development. Together with TRF8, it plays an essential role in macrophage and dendritic cell development and function. IRF4 is also reported to be essential for pre-B cell development, receptor editing, germinal center reactor and plasma cell differentiation.

Reactivity

Human, Mouse

Host

Rat

Conjugate

Yellow dye

Antibody clone number

IRF4.3E4

Vial size

25 µg

Concentration

0.2 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PHA-stimulated (3 days) or freshly isolated human peripheral lymphocytes were stained with CD4 APC, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601), and then stained with IRF4 (clone IRF4.3E4) PE (open histogram with solid line for fresh cells, filled histogram for stimulated cells) or rat IgG2a, κ PE isotype control (dash line for fresh cell, dot line for stimulated cells). Data shown was gated on CD4+ lymphocyte population.

Conjugate

Yellow dye