Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 646406 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#646406, RRID:AB_2563267
- Product name
- Alexa Fluor® 488 anti-IRF4
- Antibody type
- Monoclonal
- Antigen
- GST fusion protein containing C-terminal of murine IRF4
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Green dye
- Antibody clone number
- IRF4.3E4
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- PHA-stimulated (3 days) or freshly isolated human peripheral lymphocytes were fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601). Cells were then stained with IRF4 (clone IRF4.3E4) Alexa Fluo® 488 (open histogram with solid line: fresh cells, filled histogram: stimulated cells) or rat IgG1, κ Alexa Fluor® 488 isotype control (dash line: fresh cell, dot line: stimulated cells).
- Conjugate
- Green dye