646407

antibody from BioLegend

Targeting: IRF4 LSIRF, MUM1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

646407

Provider

BioLegend

Proper citation

BioLegend Cat#646407, RRID:AB_2564047

Product name

Alexa Fluor® 647 anti-IRF4

Antibody type

Monoclonal

Antigen

GST fusion protein containing C-terminal of murine IRF4

Reactivity

Human, Mouse

Host

Rat

Conjugate

Red dye

Antibody clone number

IRF4.3E4

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PHA-stimulated (3 days) or freshly isolated human peripheral lymphocytes were fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601). Cells were then stained with IRF4 (clone IRF4.3E4) Alexa Fluor® 647(open histogram with solid line: fresh cells, filled histogram: stimulated cells) or rat IgG1, κ Alexa Fluor® 647 isotype control (dash line: fresh cell, dot line: stimulated cells).

Conjugate

Red dye