MA5-16382

IRF4 antibody from Invitrogen Antibodies

LSIRF, MUM1

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Flow cytometry

Recommended by provider

Provider product page for MA5-16382

Antibody Data

Product number

MA5-16382

Provider

Invitrogen Antibodies

Product name

Anti-IRF4 Monoclonal Antibody (SP114)

Provider product page

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is Hodgkin's lymphoma.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

SP114

Vial size

500 µL

Concentration

Lot Dependent

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-IRF4 Monoclonal Antibody (SP114) (Product # MA5-16382) and a 51kDa band corresponding to IRF4 was observed in IM9 and was absent in MOLT-4, Jurkat and U-937 as reported. Whole cell extracts (30 µg lysate) of IM9 (Lane 1), MOLT-4 (Lane 2), Jurkat (Lane 3) and U-937 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:100 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Ramos Cells using anti-MUM1/IRF4 Monoclonal Antibody (Product # MA5-16382). The recommened dilution for this antibody in western blot applications is 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MUM1/IRF4 using anti-MUM1/IRF4 Monoclonal Antibody (Product # MA5-16382) in Hodgkin's Lymphoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MUM1/IRF4 using anti-MUM1/IRF4 Monoclonal Antibody (Product # MA5-16382) in Hodgkin's Lymphoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.