Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB5117 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IRF8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IRF8 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IRF4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q02556
- Isotype
- IgG
- Antibody clone number
- 809926
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IRF8 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. Gels were loaded with 25 µg of nuclear extracts (Nuc) and whole cell lysate (WCL). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IRF8 Monoclonal Antibody (Catalog # MAB5117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF8 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IRF8 by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for IRF8 at approximately 54 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human IRF8 Monoclonal Antibody (Catalog # MAB5117). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IRF8 in Human Tonsil. IRF8 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human IRF8 Monoclonal Antibody (Catalog # MAB5117) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.