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- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 13528-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13528-1-AP, RRID:AB_2303299
- Product name
- TDRKH specific antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated TDRKH specific antibody (Cat. #13528-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references piRNA loading triggers MIWI translocation from the intermitochondrial cement to chromatoid body during mouse spermatogenesis.
Three RNA-binding protein signature as a novel biomarker to predict endometrial cancer prognosis.
MIWI N-terminal RG motif promotes efficient pachytene piRNA production and spermatogenesis independent of LINE1 transposon silencing.
PNLDC1 catalysis and postnatal germline function are required for piRNA trimming, LINE1 silencing, and spermatogenesis in mice.
Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.
MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.
PIWIL3 Forms a Complex with TDRKH in Mammalian Oocytes.
Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors.
MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA.
Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis.
High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens.
Systematic In-Depth Proteomic Analysis of Mitochondria-Associated Endoplasmic Reticulum Membranes in Mouse and Human Testes.
PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.
Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline.
Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi.
Wei H, Gao J, Lin DH, Geng R, Liao J, Huang TY, Shang G, Jing J, Fan ZW, Pan D, Yin ZQ, Li T, Liu X, Zhao S, Chen C, Li J, Wang X, Ding D, Liu MF
Nature communications 2024 Mar 15;15(1):2343
Nature communications 2024 Mar 15;15(1):2343
Three RNA-binding protein signature as a novel biomarker to predict endometrial cancer prognosis.
Wang B, Ma X
American journal of translational research 2023;15(1):476-492
American journal of translational research 2023;15(1):476-492
MIWI N-terminal RG motif promotes efficient pachytene piRNA production and spermatogenesis independent of LINE1 transposon silencing.
Wei C, Jing J, Yan X, Mann JM, Geng R, Xie H, Demireva EY, Hess RA, Ding D, Chen C
PLoS genetics 2023 Nov;19(11):e1011031
PLoS genetics 2023 Nov;19(11):e1011031
PNLDC1 catalysis and postnatal germline function are required for piRNA trimming, LINE1 silencing, and spermatogenesis in mice.
Wei C, Yan X, Mann JM, Geng R, Xie H, Demireva EY, Sun L, Ding D, Chen C
bioRxiv : the preprint server for biology 2023 Dec 27;
bioRxiv : the preprint server for biology 2023 Dec 27;
Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.
Antico O, Ordureau A, Stevens M, Singh F, Nirujogi RS, Gierlinski M, Barini E, Rickwood ML, Prescott A, Toth R, Ganley IG, Harper JW, Muqit MMK
Science advances 2021 Nov 12;7(46):eabj0722
Science advances 2021 Nov 12;7(46):eabj0722
MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.
Wang X, Wen Y, Zhang J, Swanson G, Guo S, Cao C, Krawetz SA, Zhang Z, Yuan S
Development (Cambridge, England) 2021 Apr 1;148(7)
Development (Cambridge, England) 2021 Apr 1;148(7)
PIWIL3 Forms a Complex with TDRKH in Mammalian Oocytes.
Tan M, Tol HTAV, Rosenkranz D, Roovers EF, Damen MJ, Stout TAE, Wu W, Roelen BAJ
Cells 2020 May 29;9(6)
Cells 2020 May 29;9(6)
Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors.
Sun YH, Zhu J, Xie LH, Li Z, Meduri R, Zhu X, Song C, Chen C, Ricci EP, Weng Z, Li XZ
Nature cell biology 2020 Feb;22(2):200-212
Nature cell biology 2020 Feb;22(2):200-212
MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA.
Hsieh CL, Xia J, Lin H
The EMBO journal 2020 Aug 17;39(16):e103614
The EMBO journal 2020 Aug 17;39(16):e103614
Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis.
Ding D, Liu J, Dong K, Melnick AF, Latham KE, Chen C
Nucleic acids research 2019 Mar 18;47(5):2594-2608
Nucleic acids research 2019 Mar 18;47(5):2594-2608
High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens.
Baumann B, Lugli G, Gao S, Zenner M, Nonn L
The Prostate 2019 Jun;79(8):840-855
The Prostate 2019 Jun;79(8):840-855
Systematic In-Depth Proteomic Analysis of Mitochondria-Associated Endoplasmic Reticulum Membranes in Mouse and Human Testes.
Wang X, Wen Y, Dong J, Cao C, Yuan S
Proteomics 2018 Jul;18(14):e1700478
Proteomics 2018 Jul;18(14):e1700478
PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.
Ding D, Liu J, Dong K, Midic U, Hess RA, Xie H, Demireva EY, Chen C
Nature communications 2017 Oct 10;8(1):819
Nature communications 2017 Oct 10;8(1):819
Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline.
Saxe JP, Chen M, Zhao H, Lin H
The EMBO journal 2013 Jul 3;32(13):1869-85
The EMBO journal 2013 Jul 3;32(13):1869-85
Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi.
Chen C, Jin J, James DA, Adams-Cioaba MA, Park JG, Guo Y, Tenaglia E, Xu C, Gish G, Min J, Pawson T
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 1;106(48):20336-41
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 1;106(48):20336-41
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse testis tissue were subjected to SDS PAGE followed by western blot with 13528-1-AP(TDRKH antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using TDRKH antibody 13528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13528-1-AP(TDRKH antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13528-1-AP(TDRKH antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
