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Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- 730053 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-TDRKH Monoclonal Antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Submitted references Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, Byram G, Collins BC, Dowdell E, Fenner M, Guo X, Hutchinson A, Kennedy JJ, Krastins B, Larsen B, Lin ZY, Lopez MF, Loppnau P, Miersch S, Nguyen T, Olsen JB, Paduch M, Ravichandran M, Seitova A, Vadali G, Vogelsang MS, Whiteaker JR, Zhong G, Zhong N, Zhao L, Aebersold R, Arrowsmith CH, Emili A, Frappier L, Gingras AC, Gstaiger M, Paulovich AG, Koide S, Kossiakoff AA, Sidhu SS, Wodak SJ, Gräslund S, Greenblatt JF, Edwards AM
Nature methods 2015 Aug;12(8):725-31
Nature methods 2015 Aug;12(8):725-31
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunoprecipitation analysis using Anti-TDR2 Recombinant Mouse Monoclonal Antibody (Product # 730053) followed by western blot detection. Immunoprecipitation was performed on HEK293 cells over expressing flag-tagged TDR2 protein. Western blot using an anti-flag antibody was performed on the raw lysate (lane 1) and immunoprecipitated protein (lane 2) to evaluate the results of Immunoprecipitation. Because detection utilizes an anti-flag antibody, the presence of multiple bands suggests multiple forms of flag-tagged TDR2 protein rather than cross-reactivity of the Anti-TDR2 Recombinant Mouse Monoclonal Antibody.
