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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- HPA018979 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA018979, RRID:AB_1845696
- Product name
- Anti-SPATC1L
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SPATC1L, Gene description: spermatogenesis and centriole associated 1-like, Alternative Gene Names: C21orf56, Validated applications: ICC, WB, Uniprot ID: Q9H0A9, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.3 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Biallelic mutations in spermatogenesis and centriole-associated 1 like (SPATC1L) cause acephalic spermatozoa syndrome and male infertility
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Antibody-based Protein Profiling of the Human Chromosome 21
Li Y, Li N, Liu W, Sha Y, Wu R, Tang Y, Zhu X, Wei X, Zhang X, Wang Y, Lu Z, Zhang F
Asian Journal of Andrology 2022;24(1):67-72
Asian Journal of Andrology 2022;24(1):67-72
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
Antibody-based Protein Profiling of the Human Chromosome 21
Uhlén M, Oksvold P, Älgenäs C, Hamsten C, Fagerberg L, Klevebring D, Lundberg E, Odeberg J, Pontén F, Kondo T, Sivertsson Å
Molecular & Cellular Proteomics 2012;11(3):M111.013458
Molecular & Cellular Proteomics 2012;11(3):M111.013458
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and SPATC1L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410249).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
- Sample type
- Human
