PAB27479

antibody from Abnova Corporation

Targeting: WDR81 CAMRQ2, FLJ33817, PPP1R166, SORF-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27479

Provider

Abnova Corporation

Product name

WDR81 polyclonal antibody

Antibody type

Polyclonal

Antigen

Recombinant protein corresponding to amino acids of human WDR81.

Reactivity

Human

Host

Rabbit

Antigen sequence

LYLESISPSSRNPASVEPTMPGTGPEWDPHGGGCP
QDDGHSGTFGSVLVGNRIQIPNDSRPENPGPLGPI
SGVGGGGLGSGSDDNALKQELPRSVHGLSGNWLAY
WQYEIGVSQQDAHFHFHQIR


Isotype

IgG

Vial size

100 μl

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with WDR81 polyclonal antibody (Cat # PAB27479) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with WDR81 polyclonal antibody (Cat # PAB27479) at 1-4 ug/mL dilutions shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with WDR81 polyclonal antibody (Cat # PAB27479) shows strong nuclear positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)