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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- MA5-27865 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-C9orf72 Monoclonal Antibody (GT779)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Positive Control: U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, rat brain Predicted Reactivity: Xenopus laevis (90%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT779
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C9orf72 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with C9orf72 monoclonal antibody (Product # MA5-27865) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C9orf72 in various tissue extracts using 50 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with C9orf72 monoclonal antibody (Product # MA5-27865) using a dilution of 1:1000 followed by HRP-conjugated anti-mouse IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of C9orf72 in paraffin-embedded human colon cancer. Sample was incubated with C9orf72 monoclonal antibody (Product # MA5-27865) using a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C9orf72 in U87-MG cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with C9orf72 monoclonal antibody (Product # MA5-27865) using a dilution of 1:250, followed by Hoechst.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of C9orf72 in SK-N-SH whole cell extract. Samples were treated with C9orf72 monoclonal antibody (Product # MA5-27865) using a dilution of 5 µg followed by anti-rabbit IgG.
