22637-1-AP

antibody from Proteintech Group

Targeting: C9orf72 DENND9, DENNL72, MGC23980

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

22637-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#22637-1-AP, RRID:AB_10953528

Product name

C9orf72 antibody

Antibody type

Polyclonal

Description

KD/KO validated C9orf72 antibody (Cat. #22637-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy.

Zhang S, Tong M, Zheng D, Huang H, Li L, Ungermann C, Pan Y, Luo H, Lei M, Tang Z, Fu W, Chen S, Liu X, Zhong Q
Nature communications 2023 Oct 11;14(1):6360

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C9ORF72 suppresses JAK-STAT mediated inflammation.

Pang W, Hu F
iScience 2023 May 19;26(5):106579

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C9orf72 functions in the nucleus to regulate DNA damage repair.

He L, Liang J, Chen C, Chen J, Shen Y, Sun S, Li L
Cell death and differentiation 2023 Mar;30(3):716-730

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A high-fidelity CRISPR-Cas13 system improves abnormalities associated with C9ORF72-linked ALS/FTD.

McCallister TX, Lim CKW, Terpstra WM, Alejandra Zeballos C M, Zhang S, Powell JE, Gaj T
bioRxiv : the preprint server for biology 2023 Dec 12;

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C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains.

Jülg J, Edbauer D, Behrends C
EMBO reports 2023 Aug 3;24(8):e55895

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The C9ORF72 repeat expansion alters neurodevelopment.

Hendricks E, Quihuis AM, Hung ST, Chang J, Dorjsuren N, Der B, Staats KA, Shi Y, Sta Maria NS, Jacobs RE, Ichida JK
Cell reports 2023 Aug 29;42(8):112983

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Reversing lysosome-ribosome circuit dysregulation mitigates C9FTD/ALS neurodegeneration and behaviors.

Ma L, Liang C, Wang J, Chang Q, Wang Y, Zhang W, Du Y, Sadan J, Chen JF
Human molecular genetics 2023 Apr 6;32(8):1252-1265

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C9orf72 knockdown alleviates hepatic insulin resistance by promoting lipophagy.

Cang X, Wang Y, Zeng J, Gao J, Yu Q, Lu C, Xu F, Lin J, Zhu J, Wang X
Biochemical and biophysical research communications 2022 Jan 15;588:15-22

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Ablation of C9orf72 together with excitotoxicity induces ALS in rats.

Dong W, Ma Y, Guan F, Zhang X, Chen W, Zhang L, Zhang L
The FEBS journal 2021 Mar;288(5):1712-1723

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C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.

Wang T, Liu H, Itoh K, Oh S, Zhao L, Murata D, Sesaki H, Hartung T, Na CH, Wang J
Cell metabolism 2021 Mar 2;33(3):531-546.e9

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Staufen1 in Human Neurodegeneration.

Paul S, Dansithong W, Figueroa KP, Gandelman M, Scoles DR, Pulst SM
Annals of neurology 2021 Jun;89(6):1114-1128

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Expression of C9orf72 hexanucleotide repeat expansion leads to formation of RNA foci and dipeptide repeat proteins but does not influence autophagy or proteasomal function in neuronal cells.

Leskelä S, Huber N, Hoffmann D, Rostalski H, Remes AM, Takalo M, Hiltunen M, Haapasalo A
Biochimica et biophysica acta. Molecular cell research 2021 Jun;1868(7):119021

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BV-2 Microglial Cells Overexpressing C9orf72 Hexanucleotide Repeat Expansion Produce DPR Proteins and Show Normal Functionality but No RNA Foci.

Rostalski H, Hietanen T, Leskelä S, Behánová A, Abdollahzadeh A, Wittrahm R, Mäkinen P, Huber N, Hoffmann D, Solje E, Remes AM, Natunen T, Takalo M, Tohka J, Hiltunen M, Haapasalo A
Frontiers in neurology 2020;11:550140

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G(4)C(2) Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.

Coyne AN, Zaepfel BL, Hayes L, Fitchman B, Salzberg Y, Luo EC, Bowen K, Trost H, Aigner S, Rigo F, Yeo GW, Harel A, Svendsen CN, Sareen D, Rothstein JD
Neuron 2020 Sep 23;107(6):1124-1140.e11

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Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72.

Zhu Q, Jiang J, Gendron TF, McAlonis-Downes M, Jiang L, Taylor A, Diaz Garcia S, Ghosh Dastidar S, Rodriguez MJ, King P, Zhang Y, La Spada AR, Xu H, Petrucelli L, Ravits J, Da Cruz S, Lagier-Tourenne C, Cleveland DW
Nature neuroscience 2020 May;23(5):615-624

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C9orf72-associated SMCR8 protein binds in the ubiquitin pathway and with proteins linked with neurological disease.

Goodier JL, Soares AO, Pereira GC, DeVine LR, Sanchez L, Cole RN, García-Pérez JL
Acta neuropathologica communications 2020 Jul 16;8(1):110

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Analysis of ALS-related proteins during herpes simplex virus-2 latent infection.

Cabrera JR, Rodríguez-Izquierdo I, Jiménez JL, Muñoz-Fernández MÁ
Journal of neuroinflammation 2020 Dec 7;17(1):371

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C9orf72/ALFA-1 controls TFEB/HLH-30-dependent metabolism through dynamic regulation of Rag GTPases.

Ji YJ, Ugolino J, Zhang T, Lu J, Kim D, Wang J
PLoS genetics 2020 Apr;16(4):e1008738

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Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.

Laflamme C, McKeever PM, Kumar R, Schwartz J, Kolahdouzan M, Chen CX, You Z, Benaliouad F, Gileadi O, McBride HM, Durcan TM, Edwards AM, Healy LM, Robertson J, McPherson PS
eLife 2019 Oct 15;8

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C9orf72 Proteins Regulate Autophagy and Undergo Autophagosomal or Proteasomal Degradation in a Cell Type-Dependent Manner.

Leskelä S, Huber N, Rostalski H, Natunen T, Remes AM, Takalo M, Hiltunen M, Haapasalo A
Cells 2019 Oct 10;8(10)

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C9ORF72-related cellular pathology in skeletal myocytes derived from ALS-patient induced pluripotent stem cells.

Lynch E, Semrad T, Belsito VS, FitzGibbons C, Reilly M, Hayakawa K, Suzuki M
Disease models & mechanisms 2019 Aug 16;12(8)

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A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.

Liu Y, Wang T, Ji YJ, Johnson K, Liu H, Johnson K, Bailey S, Suk Y, Lu YN, Liu M, Wang J
Genes & development 2018 Nov 1;32(21-22):1380-1397

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Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.

Shi Y, Lin S, Staats KA, Li Y, Chang WH, Hung ST, Hendricks E, Linares GR, Wang Y, Son EY, Wen X, Kisler K, Wilkinson B, Menendez L, Sugawara T, Woolwine P, Huang M, Cowan MJ, Ge B, Koutsodendris N, Sandor KP, Komberg J, Vangoor VR, Senthilkumar K, Hennes V, Seah C, Nelson AR, Cheng TY, Lee SJ, August PR, Chen JA, Wisniewski N, Hanson-Smith V, Belgard TG, Zhang A, Coba M, Grunseich C, Ward ME, van den Berg LH, Pasterkamp RJ, Trotti D, Zlokovic BV, Ichida JK
Nature medicine 2018 Mar;24(3):313-325

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Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.

Saberi S, Stauffer JE, Jiang J, Garcia SD, Taylor AE, Schulte D, Ohkubo T, Schloffman CL, Maldonado M, Baughn M, Rodriguez MJ, Pizzo D, Cleveland D, Ravits J
Acta neuropathologica 2018 Mar;135(3):459-474

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Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.

Davidson YS, Robinson AC, Rollinson S, Pickering-Brown S, Xiao S, Robertson J, Mann DMA
Amyotrophic lateral sclerosis & frontotemporal degeneration 2018 Feb;19(1-2):102-111

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Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Frick P, Sellier C, Mackenzie IRA, Cheng CY, Tahraoui-Bories J, Martinat C, Pasterkamp RJ, Prudlo J, Edbauer D, Oulad-Abdelghani M, Feederle R, Charlet-Berguerand N, Neumann M
Acta neuropathologica communications 2018 Aug 3;6(1):72

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C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.

Maharjan N, Künzli C, Buthey K, Saxena S
Molecular neurobiology 2017 May;54(4):3062-3077

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The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.

Sullivan PM, Zhou X, Robins AM, Paushter DH, Kim D, Smolka MB, Hu F
Acta neuropathologica communications 2016 May 18;4(1):51

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C9orf72 is required for proper macrophage and microglial function in mice.

O'Rourke JG, Bogdanik L, Yáñez A, Lall D, Wolf AJ, Muhammad AK, Ho R, Carmona S, Vit JP, Zarrow J, Kim KJ, Bell S, Harms MB, Miller TM, Dangler CA, Underhill DM, Goodridge HS, Lutz CM, Baloh RH
Science (New York, N.Y.) 2016 Mar 18;351(6279):1324-9

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Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.

Sellier C, Campanari ML, Julie Corbier C, Gaucherot A, Kolb-Cheynel I, Oulad-Abdelghani M, Ruffenach F, Page A, Ciura S, Kabashi E, Charlet-Berguerand N
The EMBO journal 2016 Jun 15;35(12):1276-97

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Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.

Burberry A, Suzuki N, Wang JY, Moccia R, Mordes DA, Stewart MH, Suzuki-Uematsu S, Ghosh S, Singh A, Merkle FT, Koszka K, Li QZ, Zon L, Rossi DJ, Trowbridge JJ, Notarangelo LD, Eggan K
Science translational medicine 2016 Jul 13;8(347):347ra93

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Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.

Ferguson R, Serafeimidou-Pouliou E, Subramanian V
Journal of anatomy 2016 Dec;229(6):871-891

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22637-1-AP(C9orf72 antibody) at dilution of 1:300

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The C9orf72 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human C9orf72. This antibody recognizes human,mouse,rat antigen. The C9orf72 antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP analysis.

Appendix

22637-1-AP.pdf

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Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-C9orf72 (IP:22637-1-AP, 3ug; Detection:22637-1-AP 1:300) with SH-SY5Y cells lysate 2000ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 22637-1-AP(C9orf72 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 22637-1-AP(C9orf72 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue