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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-31565 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-C9orf72 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-31565 targets C9orf72 in IHC (P), ICC/IF, and WB applications and shows reactivity with Human and mouse samples. The PA5-31565 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 198 of Human C9orf72.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C9orf72 using 30µg of Neuro2A lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a C9orf72 polyclonal antibody (Product # PA5-31565) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C9orf72 using 30µg of U87-MG lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a C9orf72 polyclonal antibody (Product # PA5-31565) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C9orf72 using 30µg of A) Non-transfected 293T lysates and B) C9orf72 transfected 293T lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a C9orf72 polyclonal antibody (Product # PA5-31565) at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C9orf72 showing staining in the cytoplasm and nucleus of U87-MG cells. U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a C9orf72 polyclonal antibody (Product # PA5-31565) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of C9orf72 in paraffin-embedded U87 xenograft using a C9orf72 polyclonal antibody (Product # PA5-31565) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of C9orf72 in paraffin-embedded mouse fore brain tissue using a C9orf72 polyclonal antibody (Product # PA5-31565) at a dilution of 1:500.
