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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 66140-1-Ig - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#66140-1-Ig, RRID:AB_2784547
- Product name
- C9orf72 antibody
- Antibody type
- Monoclonal
- Description
- C9orf72 antibody (Cat. #66140-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 3D2H6
- Vial size
- 20ul, 150ul
Submitted references Dual-gRNA approach with limited off-target effect corrects C9ORF72 repeat expansion in vivo.
Knock in of a hexanucleotide repeat expansion in the C9orf72 gene induces ALS in rats.
Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.
Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.
Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.
C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.
Piao X, Meng D, Zhang X, Song Q, Lv H, Jia Y
Scientific reports 2022 Apr 5;12(1):5672
Scientific reports 2022 Apr 5;12(1):5672
Knock in of a hexanucleotide repeat expansion in the C9orf72 gene induces ALS in rats.
Dong W, Zhang L, Sun C, Gao X, Guan F, Li J, Chen W, Ma Y, Zhang L
Animal models and experimental medicine 2020 Sep;3(3):237-244
Animal models and experimental medicine 2020 Sep;3(3):237-244
Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.
Saberi S, Stauffer JE, Jiang J, Garcia SD, Taylor AE, Schulte D, Ohkubo T, Schloffman CL, Maldonado M, Baughn M, Rodriguez MJ, Pizzo D, Cleveland D, Ravits J
Acta neuropathologica 2018 Mar;135(3):459-474
Acta neuropathologica 2018 Mar;135(3):459-474
Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.
Davidson YS, Robinson AC, Rollinson S, Pickering-Brown S, Xiao S, Robertson J, Mann DMA
Amyotrophic lateral sclerosis & frontotemporal degeneration 2018 Feb;19(1-2):102-111
Amyotrophic lateral sclerosis & frontotemporal degeneration 2018 Feb;19(1-2):102-111
Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.
Frick P, Sellier C, Mackenzie IRA, Cheng CY, Tahraoui-Bories J, Martinat C, Pasterkamp RJ, Prudlo J, Edbauer D, Oulad-Abdelghani M, Feederle R, Charlet-Berguerand N, Neumann M
Acta neuropathologica communications 2018 Aug 3;6(1):72
Acta neuropathologica communications 2018 Aug 3;6(1):72
C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.
Sivadasan R, Hornburg D, Drepper C, Frank N, Jablonka S, Hansel A, Lojewski X, Sterneckert J, Hermann A, Shaw PJ, Ince PG, Mann M, Meissner F, Sendtner M
Nature neuroscience 2016 Dec;19(12):1610-1618
Nature neuroscience 2016 Dec;19(12):1610-1618
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 66140-1-Ig(C9orf72 antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The C9orf72 antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human C9orf72. This antibody recognizes human,mouse,rat antigen. The C9orf72 antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-C9orf72 (IP:66140-1-Ig, 4ug; Detection:66140-1-Ig 1:1000) with C6 cells lysate 1320ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
