Antibody data
- Antibody Data
- Antigen structure
- References [60]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
Reference
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- Product number
- 55028-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#55028-1-AP, RRID:AB_10858390
- Product name
- PFKM antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated PFKM antibody (Cat. #55028-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Glutaminase potentiates the glycolysis in esophageal squamous cell carcinoma by interacting with PDK1.
Icariin ameliorates glycolytic dysfunction in Alzheimer's disease models by activating the Wnt/β-catenin signaling pathway.
GL-V9 inhibits the activation of AR-AKT-HK2 signaling networks and induces prostate cancer cell apoptosis through mitochondria-mediated mechanism.
Physiological and molecular predictors of cycling sprint performance.
Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands.
Tripartite-motif 3 represses ovarian cancer progression by downregulating lactate dehydrogenase A and inhibiting AKT signaling.
The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity.
Determinants of the maximal functional reserve during repeated supramaximal exercise by humans: The roles of Nrf2/Keap1, antioxidant proteins, muscle phenotype and oxygenation.
Emodin promotes hepatic stellate cell senescence and alleviates liver fibrosis via a nuclear receptor (Nur77)-mediated epigenetic regulation of glutaminase 1.
Depressed Proximal Glycolysis in Myocardium Of Human Heart Failure with Preserved Ejection Fraction.
Radiation-Induced Changes in Energy Metabolism Result in Mitochondrial Dysfunction in Salivary Glands.
Ectopic endometriotic stromal cells-derived lactate induces M2 macrophage polarization via Mettl3/Trib1/ERK/STAT3 signalling pathway in endometriosis.
Aging Is Associated With Impaired Postprandial Response of Skeletal Muscle Protein Synthesis to High-Intensity Muscle Contraction in Mice.
Lactate metabolism is essential in early-onset mitochondrial myopathy.
The Metabolic Changes between Monolayer (2D) and Three-Dimensional (3D) Culture Conditions in Human Mesenchymal Stem/Stromal Cells Derived from Adipose Tissue.
AMPK regulates homeostasis of invasion and viability in trophoblasts by redirecting glucose metabolism: Implications for pre-eclampsia.
Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P.
Cyclin D1 extensively reprograms metabolism to support biosynthetic pathways in hepatocytes.
Effect of chronic heat stress on the carbonylation of glycolytic enzymes in breast muscle and its correlation with the growth performance of broilers.
RPLP2 activates TLR4 in an autocrine manner and promotes HIF-1α-induced metabolic reprogramming in hepatocellular carcinoma.
Exosome-Related FTCD Facilitates M1 Macrophage Polarization and Impacts the Prognosis of Hepatocellular Carcinoma.
Huangqi-Danshen decoction reshapes renal glucose metabolism profiles that delays chronic kidney disease progression.
Aspirin induces immunogenic cell death and enhances cancer immunotherapy in colorectal cancer.
Coxsackievirus B3 infection induces glycolysis to facilitate viral replication.
A Photoannealed Granular Hydrogel Facilitating Hyaline Cartilage Regeneration via Improving Chondrogenic Phenotype.
Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis.
Effect of endurance training and PGC-1α overexpression on calculated lactate production volume during exercise based on blood lactate concentration.
Paeoniflorin ameliorates airway inflammation and immune response in ovalbumin induced asthmatic mice: From oxidative stress to autophagy.
GCN5-mediated PKM2 acetylation participates in benzene-induced hematotoxicity through regulating glycolysis and inflammation via p-Stat3/IL17A axis.
Disrupting Circadian Rhythm via the PER1-HK2 Axis Reverses Trastuzumab Resistance in Gastric Cancer.
Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase via Activation of PDE5A-cGMP-PKG in Cattle Heart.
Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.
Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.
Relocation of phosphofructokinases within epithelial cells is a novel event preceding breast cancer recurrence that accurately predicts patient outcomes.
The alterations of miRNA and mRNA expression profile and their integration analysis induced by silica nanoparticles in spermatocyte cells.
CTPS and IMPDH form cytoophidia in developmental thymocytes.
The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.
Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.
L-Theanine affects intestinal mucosal immunity by regulating short-chain fatty acid metabolism under dietary fiber feeding.
A glycolytic shift in Schwann cells supports injured axons.
Synthesis and biological evaluation of novel 5,6,7-trimethoxy flavonoid salicylate derivatives as potential anti-tumor agents.
Mechanical regulation of glycolysis via cytoskeleton architecture.
Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.
Protein recycling and limb muscle recovery after critical illness in slow- and fast-twitch limb muscle.
Click RNA for Rapid Capture and Identification of Intracellular MicroRNA Targets.
Mutant IDH1 confers resistance to energy stress in normal biliary cells through PFKP-induced aerobic glycolysis and AMPK activation.
Energy Metabolism in the Bone is Associated with Histomorphometric Changes in Rats with Hyperthyroidism.
High salt diet induces metabolic alterations in multiple biological processes of Dahl salt-sensitive rats.
Resveratrol inhibits VEGF-induced angiogenesis in human endothelial cells associated with suppression of aerobic glycolysis via modulation of PKM2 nuclear translocation.
Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.
Dahuang Zhechong Pill Combined with Doxorubicin Induces Cell Death through Regulating Energy Metabolism in Human Hepatocellular Carcinoma Cells.
A large-scale targeted proteomics assay resource based on an in vitro human proteome.
CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.
Testosterone differentially regulates targets of lipid and glucose metabolism in liver, muscle and adipose tissues of the testicular feminised mouse.
The platelet isoform of phosphofructokinase contributes to metabolic reprogramming and maintains cell proliferation in clear cell renal cell carcinoma.
Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.
Poly(ADP-ribose) polymerase regulates glycolytic activity in kidney proximal tubule epithelial cells.
The drs tumor suppressor regulates glucose metabolism via lactate dehydrogenase-B.
Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.
ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.
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Tripartite-motif 3 represses ovarian cancer progression by downregulating lactate dehydrogenase A and inhibiting AKT signaling.
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The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity.
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Determinants of the maximal functional reserve during repeated supramaximal exercise by humans: The roles of Nrf2/Keap1, antioxidant proteins, muscle phenotype and oxygenation.
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Emodin promotes hepatic stellate cell senescence and alleviates liver fibrosis via a nuclear receptor (Nur77)-mediated epigenetic regulation of glutaminase 1.
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Ectopic endometriotic stromal cells-derived lactate induces M2 macrophage polarization via Mettl3/Trib1/ERK/STAT3 signalling pathway in endometriosis.
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AMPK regulates homeostasis of invasion and viability in trophoblasts by redirecting glucose metabolism: Implications for pre-eclampsia.
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Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P.
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Effect of chronic heat stress on the carbonylation of glycolytic enzymes in breast muscle and its correlation with the growth performance of broilers.
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Aspirin induces immunogenic cell death and enhances cancer immunotherapy in colorectal cancer.
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Coxsackievirus B3 infection induces glycolysis to facilitate viral replication.
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A Photoannealed Granular Hydrogel Facilitating Hyaline Cartilage Regeneration via Improving Chondrogenic Phenotype.
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ACS applied materials & interfaces 2022 Sep 14;14(36):40674-40687
Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis.
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iScience 2022 Nov 18;25(11):105353
Effect of endurance training and PGC-1α overexpression on calculated lactate production volume during exercise based on blood lactate concentration.
Takeda R, Nonaka Y, Kakinoki K, Miura S, Kano Y, Hoshino D
Scientific reports 2022 Jan 31;12(1):1635
Scientific reports 2022 Jan 31;12(1):1635
Paeoniflorin ameliorates airway inflammation and immune response in ovalbumin induced asthmatic mice: From oxidative stress to autophagy.
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Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Feb;96:153835
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Feb;96:153835
GCN5-mediated PKM2 acetylation participates in benzene-induced hematotoxicity through regulating glycolysis and inflammation via p-Stat3/IL17A axis.
Zhang W, Guo X, Ren J, Chen Y, Wang J, Gao A
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Disrupting Circadian Rhythm via the PER1-HK2 Axis Reverses Trastuzumab Resistance in Gastric Cancer.
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Cancer research 2022 Apr 15;82(8):1503-1517
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Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase via Activation of PDE5A-cGMP-PKG in Cattle Heart.
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Frontiers in cell and developmental biology 2021;9:774185
Frontiers in cell and developmental biology 2021;9:774185
Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.
Yang K, Wu K, Feng J, Yutian L, Zhu X, Xu D
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Evidence-based complementary and alternative medicine : eCAM 2021;2021:5526581
Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.
Chen X, Sun N, Li R, Sang X, Li X, Zhao J, Han J, Yang J, Ikezoe T
International journal of biological sciences 2021;17(5):1263-1276
International journal of biological sciences 2021;17(5):1263-1276
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American journal of physiology. Cell physiology 2021 Oct 1;321(4):C654-C670
The alterations of miRNA and mRNA expression profile and their integration analysis induced by silica nanoparticles in spermatocyte cells.
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CTPS and IMPDH form cytoophidia in developmental thymocytes.
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Experimental cell research 2021 Aug 1;405(1):112662
Experimental cell research 2021 Aug 1;405(1):112662
The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.
Gao W, Huang M, Chen X, Chen J, Zou Z, Li L, Ji K, Nie Z, Yang B, Wei Z, Xu P, Jia J, Zhang Q, Shen H, Wang Q, Li K, Zhu L, Wang M, Ye S, Zeng S, Lin Y, Rong Z, Xu Y, Zhu P, Zhang H, Hao B, Liu Q
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Cell death & disease 2021 Apr 15;12(4):408
Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.
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Oxidative medicine and cellular longevity 2020;2020:5849794
Oxidative medicine and cellular longevity 2020;2020:5849794
L-Theanine affects intestinal mucosal immunity by regulating short-chain fatty acid metabolism under dietary fiber feeding.
Xu W, Lin L, Liu A, Zhang T, Zhang S, Li Y, Chen J, Gong Z, Liu Z, Xiao W
Food & function 2020 Sep 23;11(9):8369-8379
Food & function 2020 Sep 23;11(9):8369-8379
A glycolytic shift in Schwann cells supports injured axons.
Babetto E, Wong KM, Beirowski B
Nature neuroscience 2020 Oct;23(10):1215-1228
Nature neuroscience 2020 Oct;23(10):1215-1228
Synthesis and biological evaluation of novel 5,6,7-trimethoxy flavonoid salicylate derivatives as potential anti-tumor agents.
Liu R, Deng X, Peng Y, Feng W, Xiong R, Zou Y, Lei X, Zheng X, Xie Z, Tang G
Bioorganic chemistry 2020 Mar;96:103652
Bioorganic chemistry 2020 Mar;96:103652
Mechanical regulation of glycolysis via cytoskeleton architecture.
Park JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen CS, Gao B, Minna JD, Bachoo R, DeBerardinis RJ, Danuser G
Nature 2020 Feb;578(7796):621-626
Nature 2020 Feb;578(7796):621-626
Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.
Wu M, Liao L, Jiang L, Zhang C, Gao H, Qiao L, Liu S, Shi D
Biomaterials 2019 Nov;222:119457
Biomaterials 2019 Nov;222:119457
Protein recycling and limb muscle recovery after critical illness in slow- and fast-twitch limb muscle.
Preau S, Ambler M, Sigurta A, Kleyman A, Dyson A, Hill NE, Boulanger E, Singer M
American journal of physiology. Regulatory, integrative and comparative physiology 2019 May 1;316(5):R584-R593
American journal of physiology. Regulatory, integrative and comparative physiology 2019 May 1;316(5):R584-R593
Click RNA for Rapid Capture and Identification of Intracellular MicroRNA Targets.
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Analytical chemistry 2019 Dec 17;91(24):15740-15747
Analytical chemistry 2019 Dec 17;91(24):15740-15747
Mutant IDH1 confers resistance to energy stress in normal biliary cells through PFKP-induced aerobic glycolysis and AMPK activation.
Fujiwara H, Tateishi K, Misumi K, Hayashi A, Igarashi K, Kato H, Nakatsuka T, Suzuki N, Yamamoto K, Kudo Y, Hayakawa Y, Nakagawa H, Tanaka Y, Ijichi H, Kogure H, Nakai Y, Isayama H, Hasegawa K, Fukayama M, Soga T, Koike K
Scientific reports 2019 Dec 11;9(1):18859
Scientific reports 2019 Dec 11;9(1):18859
Energy Metabolism in the Bone is Associated with Histomorphometric Changes in Rats with Hyperthyroidism.
Hu Z, Du M, Lai W, Liang Y, Liu Q, Mo Y, Bei J, Li S, Yang Y, Xu J, Cui L
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(4):1471-1482
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(4):1471-1482
High salt diet induces metabolic alterations in multiple biological processes of Dahl salt-sensitive rats.
Wang Y, Liu X, Zhang C, Wang Z
The Journal of nutritional biochemistry 2018 Jun;56:133-141
The Journal of nutritional biochemistry 2018 Jun;56:133-141
Resveratrol inhibits VEGF-induced angiogenesis in human endothelial cells associated with suppression of aerobic glycolysis via modulation of PKM2 nuclear translocation.
Wu H, He L, Shi J, Hou X, Zhang H, Zhang X, An Q, Fan F
Clinical and experimental pharmacology & physiology 2018 Dec;45(12):1265-1273
Clinical and experimental pharmacology & physiology 2018 Dec;45(12):1265-1273
Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.
Wu L, Cao KX, Ni ZH, Li WD, Chen ZP, Cheng HB, Liu X
Journal of ethnopharmacology 2018 Aug 10;222:71-78
Journal of ethnopharmacology 2018 Aug 10;222:71-78
Dahuang Zhechong Pill Combined with Doxorubicin Induces Cell Death through Regulating Energy Metabolism in Human Hepatocellular Carcinoma Cells.
Wu L, Zhao J, Cai H, Wang J, Chen Z, Li W, Liu X
Evidence-based complementary and alternative medicine : eCAM 2017;2017:6279576
Evidence-based complementary and alternative medicine : eCAM 2017;2017:6279576
A large-scale targeted proteomics assay resource based on an in vitro human proteome.
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Nature methods 2017 Mar;14(3):251-258
Nature methods 2017 Mar;14(3):251-258
CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.
Gu Z, Zhang H, Li Y, Shen S, Yin X, Zhang W, Cheng R, Zhang Y, Zhang X, Chen H, Huang B, Cao Y
Oncotarget 2017 Dec 26;8(69):114328-114343
Oncotarget 2017 Dec 26;8(69):114328-114343
Testosterone differentially regulates targets of lipid and glucose metabolism in liver, muscle and adipose tissues of the testicular feminised mouse.
Kelly DM, Akhtar S, Sellers DJ, Muraleedharan V, Channer KS, Jones TH
Endocrine 2016 Nov;54(2):504-515
Endocrine 2016 Nov;54(2):504-515
The platelet isoform of phosphofructokinase contributes to metabolic reprogramming and maintains cell proliferation in clear cell renal cell carcinoma.
Wang J, Zhang P, Zhong J, Tan M, Ge J, Tao L, Li Y, Zhu Y, Wu L, Qiu J, Tong X
Oncotarget 2016 May 10;7(19):27142-57
Oncotarget 2016 May 10;7(19):27142-57
Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.
Li P, Zhang D, Shen L, Dong K, Wu M, Ou Z, Shi D
Scientific reports 2016 Mar 9;6:22831
Scientific reports 2016 Mar 9;6:22831
Poly(ADP-ribose) polymerase regulates glycolytic activity in kidney proximal tubule epithelial cells.
Song H, Yoon SP, Kim J
Anatomy & cell biology 2016 Jun;49(2):79-87
Anatomy & cell biology 2016 Jun;49(2):79-87
The drs tumor suppressor regulates glucose metabolism via lactate dehydrogenase-B.
Tambe Y, Hasebe M, Kim CJ, Yamamoto A, Inoue H
Molecular carcinogenesis 2016 Jan;55(1):52-63
Molecular carcinogenesis 2016 Jan;55(1):52-63
Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.
Dong K, Wu M, Liu X, Huang Y, Zhang D, Wang Y, Yan LJ, Shi D
Free radical biology & medicine 2016 Dec;101:334-347
Free radical biology & medicine 2016 Dec;101:334-347
ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.
Dong K, Ni H, Wu M, Tang Z, Halim M, Shi D
Biochemical and biophysical research communications 2016 Aug 5;476(4):204-211
Biochemical and biophysical research communications 2016 Aug 5;476(4):204-211
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55028-1-AP(PFKM antibody) at dilution of 1:800
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The PFKM antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human PFKM. This antibody recognizes human,mouse,rat antigen. The PFKM antibody has been validated for the following applications: ELISA, WB, IF, IHC analysis.