MA5-34644

antibody from Invitrogen Antibodies

Targeting: PFKM PFK-1, PFKX, PPP1R122

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34644

Provider

Invitrogen Antibodies

Product name

PFKM Recombinant Rabbit Monoclonal Antibody (JU53-31)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: PC-3M, LOVO, SH-SY-5Y, rat brain tissue, human liver cancer tissue, human thyroid gland tissue, mouse skeletal muscle tissue, human kidney tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JU53-31

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Fructose 6 Phosphate Kinase in PC-3M cell lysate. Samples were incubated with Fructose 6 Phosphate Kinase monoclonal antibody (Product # MA5-34644), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Fructose 6 Phosphate Kinase in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Fructose 6 Phosphate Kinase monoclonal antibody (Product # MA5-34644), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Fructose 6 Phosphate Kinase in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Fructose 6 Phosphate Kinase monoclonal antibody (Product # MA5-34644), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Fructose 6 Phosphate Kinase in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Fructose 6 Phosphate Kinase monoclonal antibody (Product # MA5-34644), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Fructose 6 Phosphate Kinase in paraffin-embedded rat brain tissue. Samples were incubated with Fructose 6 Phosphate Kinase monoclonal antibody (Product # MA5-34644), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Fructose 6 Phosphate Kinase in paraffin-embedded mouse skeletal muscle tissue. Samples were incubated with Fructose 6 Phosphate Kinase monoclonal antibody (Product # MA5-34644), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Fructose 6 Phosphate Kinase in paraffin-embedded human thyroid gland tissue. Samples were incubated with Fructose 6 Phosphate Kinase monoclonal antibody (Product # MA5-34644), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Fructose 6 Phosphate Kinase in paraffin-embedded human liver cancer tissue. Samples were incubated with Fructose 6 Phosphate Kinase monoclonal antibody (Product # MA5-34644), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Fructose 6 Phosphate Kinase in paraffin-embedded human kidney tissue. Samples were incubated with Fructose 6 Phosphate Kinase monoclonal antibody (Product # MA5-34644), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Fructose 6 Phosphate Kinase in SH-SY-5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Fructose 6 Phosphate Kinase monoclonal antibody (Product # MA5-34644) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.