Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27319 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WRN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431.
- Concentration
- 1 mg/mL
Submitted references Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling.
Ponsioen B, Post JB, Buissant des Amorie JR, Laskaris D, van Ineveld RL, Kersten S, Bertotti A, Sassi F, Sipieter F, Cappe B, Mertens S, Verlaan-Klink I, Boj SF, Vries RGJ, Rehmann H, Vandenabeele P, Riquet FB, Trusolino L, Bos JL, Snippert HJG
Nature cell biology 2021 Apr;23(4):377-390
Nature cell biology 2021 Apr;23(4):377-390
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using WRN Polyclonal Antibody (Product # PA5-27319). Sample (30 µg of whole cell lysate). Lane A: A431 . 5% SDS PAGE. WRN Polyclonal Antibody (Product # PA5-27319) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of WRN in paraformaldehyde-fixed HeLa cells using a WRN polyclonal antibody (Product # PA5-27319) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WRN Polyclonal Antibody detects WRN protein at nucleus on human normal colon by immunohistochemical analysis. Sample: Paraffin-embedded human normal colon. WRN Polyclonal Antibody (Product # PA5-27319) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
