LS-C204589

antibody from LSBio

Targeting: UCHL1 PARK5, PGP9.5, Uch-L1

Western blot (Enhanced validation)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C204589

Provider

LSBio

Product name

UCHL1 / PGP9.5 Antibody (clone BH7) LS-C204589

Antibody type

Monoclonal

Description

Ascites

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

BH7

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Blots of whole cell homogenate of bovine brain stained with UCHL1 / PGP9.5 antibody (right most lane) and two other monoclonal antibodies reactive with UCHL1 (left and central lane). All three antibodies show a single clean band running at about 24kDa.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Shows a section of rat spinal cord stained with UCHL1 clone UCHL1 / PGP9.5 antibody (red) and rabbit antibody to neurofilament NF-H (green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites. The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large motor neurons.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Shows a section of rat spinal cord stained with UCHL1 clone UCHL1 / PGP9.5 antibody (red) and rabbit antibody to neurofilament NF-H (green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites. The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large motor neurons.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Shows a section of rat spinal cord stained with UCHL1 clone UCHL1 / PGP9.5 antibody (red) and rabbit antibody to neurofilament NF-H (green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites. The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large motor neurons.