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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- LS-C99517 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C99517, RRID:AB_2210640
- Product name
- UCHL1 / PGP9.5 Antibody (aa16-46) LS-C99517
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of UCHL1 (arrow) using rabbit polyclonal UCHL1-V31. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCHL1 gene (Lane 2) (Origene Technologies).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of UCHL1-V31 in CEM(lane 1), Jurkat(lane 2), Y79(lane 3) cell line and mouse brain tissue(lane 4) lysates (35 ug/lane). UCHL1 (arrow) was detected using the purified antibody.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
