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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- AM1959b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1959b, RRID:AB_11134666
- Product name
- UCHL1 Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.32 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of lysates from U266, mouse Neuro-2a, rat PC-12, C6 cell line and mouse brain tissue lysate(from left to right), using UCHL1 Antibody(Cat. #AM1959b). AM1959b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- UCHL1 Antibody(Cat. #AM1959b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).
- Primary Ab dilution
- 1:120~1000
