Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F47951 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PGP9.5 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 16-46 from the human protein was used as the immunogen for this PGP9.5 antibody.
- Description
- Purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the PGP9.5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of PGP9.5 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCHL1 gene (2).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of PGP9.5 antibody and CEM (lane 1), Jurkat (2), Y79 (3) cell lines and mouse brain tissue (4) lysate. Predicted molecular weight ~25 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PGP9.5 antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human brain tissue stained with PGP9.5 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- PGP9.5 antibody flow cytometric analysis of NCI-H460 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.