Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM09027PU-N - Provider product page
- Provider
- OriGene
- Product name
- PGP9.5 (UCHL1) mouse monoclonal antibody, clone 3D9, Purified
- Antibody type
- Monoclonal
- Description
- PGP9.5 (UCHL1) mouse monoclonal antibody, clone 3D9, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- UCHL1
- Isotype
- IgG
- Antibody clone number
- 3D9
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: The extracts of mouse brain (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human PGP9.5 Antibody at 1/1000. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: Paraffin embedded sections of human nerve tissue were incubated with anti-Human PGP9.5 Antibody at 1/100 for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
- Validation comment
- IHC