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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00007345-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007345-M01, RRID:AB_425734
- Product name
- UCHL1 monoclonal antibody (M01), clone 1B8-4D2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant UCHL1.
- Antigen sequence
MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLE
EESLGSVPAPACALLLLFPLTAQHENFRKKQIEEL
KGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDK
LGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQ
AAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYEL
DGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQG
EVRFSAVALCKAA- Isotype
- IgG
- Antibody clone number
- 1B8-4D2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- UCHL1 monoclonal antibody (M01), clone 1B8-4D2 Western Blot analysis of UCHL1 expression in IMR-32 ( Cat # L008V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of UCHL1 expression in transfected 293T cell line by UCHL1 monoclonal antibody (M01), clone 1B8-4D2.Lane 1: UCHL1 transfected lysate (Predicted MW: 24.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged UCHL1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to UCHL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ~ 10 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
