Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-87334 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87334, RRID:AB_11011000
- Product name
- Rabbit Polyclonal UCH-L1/PGP9.5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat UCH-L1/PGP9.5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The prognostic potential and oncogenic effects of PRR11 expression in hilar cholangiocarcinoma.
Antibody-based proteomics for discovery and exploration of proteins expressed in pancreatic islets.
Chen Y, Cha Z, Fang W, Qian B, Yu W, Li W, Yu G, Gao Y
Oncotarget 2015 Aug 21;6(24):20419-33
Oncotarget 2015 Aug 21;6(24):20419-33
Antibody-based proteomics for discovery and exploration of proteins expressed in pancreatic islets.
Lindskog C, Asplund A, Engkvist M, Uhlen M, Korsgren O, Ponten F
Discovery medicine 2010 Jun;9(49):565-78
Discovery medicine 2010 Jun;9(49):565-78
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in human cerebral cortex tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglion and peripheral nerves.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons and in neuropil.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human liver shows no positivity in hepatocytes, as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP1-87334] - Staining in human cerebral cortex and liver tissues . Corresponding UCHL1 RNA-seq data are presented for the same tissues.