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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA1-41464 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGP9.5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PGP9.5 in human brain lysate in the 1) absence and 2) presence of immunizing peptide. Samples were incubated in PGP9.5 polyclonal antibody (Product # PA1-41464) using a dilution of 0.2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PGP9.5 Polyclonal Antibody (Product # PA1-41464) and a 24 kDa band corresponding to PGP9.5 was observed in cell lines and tissues. Whole Cell Extract-WCL (30 µg lysate) of DU 145 (Lane 1), SH-SY5Y (Lane 2), K-562 (Lane 3), HeLa (Lane 4), Hep G2 (Lane 5), Neuro-2a (Lane 6), Rat Brain (Lane 7), Rat Liver (Lane 8), Rat Heart (Lane 9), Mouse Brain (Lane 10) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PGP9.5 in human brain, cortex. Samples were incubated in PGP9.5 polyclonal antibody (Product # PA1-41464) using a dilution of 10 µg/mL.
