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- References [24]
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- Immunohistochemistry [1]
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- Product number
- HPA005993 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA005993, RRID:AB_1858560
- Product name
- Anti-UCHL1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human UCHL1, Gene description: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), Alternative Gene Names: PARK5, PGP9.5, Uch-L1, Validated applications: ICC, IHC, WB, Uniprot ID: P09936, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.3 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Small Cell Lung Cancer Plasticity Enables NFIB-Independent Metastasis
A multiplexed in vivo approach to identify driver genes in small cell lung cancer
Targeted epigenetic silencing of UCHL1 expression suppresses collagen-1 production in human lung epithelial cells
A Novel Subgroup of UCHL1-Related Cancers Is Associated with Genomic Instability and Sensitivity to DNA-Damaging Treatment
Fully accessible fitness landscape of oncogene-negative lung adenocarcinoma
Histopathologic and proteogenomic heterogeneity reveals features of clear cell renal cell carcinoma aggressiveness.
Combinatorial Inactivation of Tumor Suppressors Efficiently Initiates Lung Adenocarcinoma with Therapeutic Vulnerabilities
A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer
Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
Genetic Determinants of EGFR-Driven Lung Cancer Growth and Therapeutic Response In Vivo
MYC Drives Temporal Evolution of Small Cell Lung Cancer Subtypes by Reprogramming Neuroendocrine Fate
Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-Terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos
Ubiquitin Carboxyl-Terminal Hydrolase L1 (UCHL1) Promotes Uterine Serous Cancer Cell Proliferation and Cell Cycle Progression
Axon-like protrusions promote small cell lung cancer migration and metastasis
Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells
Crebbp Loss Drives Small Cell Lung Cancer and Increases Sensitivity to HDAC Inhibition
Intertumoral Heterogeneity in SCLC Is Influenced by the Cell Type of Origin
UCHL1-HIF-1 axis-mediated antioxidant property of cancer cells as a therapeutic target for radiosensitization
Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer
Genetic requirement forMycland efficacy of RNA Pol I inhibition in mouse models of small cell lung cancer
The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin
The prognostic potential and oncogenic effects of PRR11 expression in hilar cholangiocarcinoma
Antibody-based proteomics for discovery and exploration of proteins expressed in pancreatic islets.
Ko J, Lambert K, Bhattacharya D, Lee M, Colón C, Hauser H, Sage J
Cancer Research 2024;84(2):226-240
Cancer Research 2024;84(2):226-240
A multiplexed in vivo approach to identify driver genes in small cell lung cancer
Lee M, Cai H, Murray C, Li C, Shue Y, Andrejka L, He A, Holzem A, Drainas A, Ko J, Coles G, Kong C, Zhu S, Zhu C, Wang J, van de Rijn M, Petrov D, Winslow M, Sage J
Cell Reports 2023;42(1):111990
Cell Reports 2023;42(1):111990
Targeted epigenetic silencing of UCHL1 expression suppresses collagen-1 production in human lung epithelial cells
Wu D, Lau A, Xu Y, Reinders-Luinge M, Koncz M, Kiss A, Timens W, Rots M, Hylkema M
Epigenetics 2023;18(1)
Epigenetics 2023;18(1)
A Novel Subgroup of UCHL1-Related Cancers Is Associated with Genomic Instability and Sensitivity to DNA-Damaging Treatment
Burkart S, Weusthof C, Khorani K, Steen S, Stögbauer F, Unger K, Hess J, Zitzelsberger H, Belka C, Kurth I, Hess J
Cancers 2023;15(6):1655
Cancers 2023;15(6):1655
Fully accessible fitness landscape of oncogene-negative lung adenocarcinoma
Yousefi M, Andrejka L, Szamecz M, Winslow M, Petrov D, Boross G
Proceedings of the National Academy of Sciences 2023;120(38)
Proceedings of the National Academy of Sciences 2023;120(38)
Histopathologic and proteogenomic heterogeneity reveals features of clear cell renal cell carcinoma aggressiveness.
Li Y, Lih TM, Dhanasekaran SM, Mannan R, Chen L, Cieslik M, Wu Y, Lu RJ, Clark DJ, Kołodziejczak I, Hong R, Chen S, Zhao Y, Chugh S, Caravan W, Naser Al Deen N, Hosseini N, Newton CJ, Krug K, Xu Y, Cho KC, Hu Y, Zhang Y, Kumar-Sinha C, Ma W, Calinawan A, Wyczalkowski MA, Wendl MC, Wang Y, Guo S, Zhang C, Le A, Dagar A, Hopkins A, Cho H, Leprevost FDV, Jing X, Teo GC, Liu W, Reimers MA, Pachynski R, Lazar AJ, Chinnaiyan AM, Van Tine BA, Zhang B, Rodland KD, Getz G, Mani DR, Wang P, Chen F, Hostetter G, Thiagarajan M, Linehan WM, Fenyö D, Jewell SD, Omenn GS, Mehra R, Wiznerowicz M, Robles AI, Mesri M, Hiltke T, An E, Rodriguez H, Chan DW, Ricketts CJ, Nesvizhskii AI, Zhang H, Ding L, Clinical Proteomic Tumor Analysis Consortium
Cancer cell 2023 Jan 9;41(1):139-163.e17
Cancer cell 2023 Jan 9;41(1):139-163.e17
Combinatorial Inactivation of Tumor Suppressors Efficiently Initiates Lung Adenocarcinoma with Therapeutic Vulnerabilities
Yousefi M, Boross G, Weiss C, Murray C, Hebert J, Cai H, Ashkin E, Karmakar S, Andrejka L, Chen L, Wang M, Tsai M, Lin W, Li C, Yakhchalian P, Colón C, Chew S, Chu P, Swanton C, Kunder C, Petrov D, Winslow M
Cancer Research 2022;82(8):1589-1602
Cancer Research 2022;82(8):1589-1602
A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
Shue Y, Drainas A, Li N, Pearsall S, Morgan D, Sinnott-Armstrong N, Hipkins S, Coles G, Lim J, Oro A, Simpson K, Dive C, Sage J
Nature Communications 2022;13(1)
Nature Communications 2022;13(1)
ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer
Olsen R, Ireland A, Kastner D, Groves S, Spainhower K, Pozo K, Kelenis D, Whitney C, Guthrie M, Wait S, Soltero D, Witt B, Quaranta V, Johnson J, Oliver T
Genes & Development 2021;35(11-12):847-869
Genes & Development 2021;35(11-12):847-869
Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
Davies C, Vidal S, Phu L, Sudhamsu J, Hinkle T, Chan Rosenberg S, Schumacher F, Zeng Y, Schwerdtfeger C, Peterson A, Lill J, Rose C, Shaw A, Wertz I, Kirkpatrick D, Koerber J
Nature Communications 2021;12(1)
Nature Communications 2021;12(1)
Genetic Determinants of EGFR-Driven Lung Cancer Growth and Therapeutic Response In Vivo
Foggetti G, Li C, Cai H, Hellyer J, Lin W, Ayeni D, Hastings K, Choi J, Wurtz A, Andrejka L, Maghini D, Rashleigh N, Levy S, Homer R, Gettinger S, Diehn M, Wakelee H, Petrov D, Winslow M, Politi K
Cancer Discovery 2021;11(7):1736-1753
Cancer Discovery 2021;11(7):1736-1753
MYC Drives Temporal Evolution of Small Cell Lung Cancer Subtypes by Reprogramming Neuroendocrine Fate
Ireland A, Micinski A, Kastner D, Guo B, Wait S, Spainhower K, Conley C, Chen O, Guthrie M, Soltero D, Qiao Y, Huang X, Tarapcsák S, Devarakonda S, Chalishazar M, Gertz J, Moser J, Marth G, Puri S, Witt B, Spike B, Oliver T
Cancer Cell 2020;38(1):60-78.e12
Cancer Cell 2020;38(1):60-78.e12
Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-Terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos
Kooij R, Liu S, Sapmaz A, Xin B, Janssen G, van Veelen P, Ovaa H, Dijke P, Geurink P
Journal of the American Chemical Society 2020;142(39):16825-16841
Journal of the American Chemical Society 2020;142(39):16825-16841
Ubiquitin Carboxyl-Terminal Hydrolase L1 (UCHL1) Promotes Uterine Serous Cancer Cell Proliferation and Cell Cycle Progression
Kwan S, Au-Yeung C, Yeung T, Rynne-Vidal A, Wong K, Risinger J, Lin H, Schmandt R, Yates M, Mok S, Lu K
Cancers 2020;12(1):118
Cancers 2020;12(1):118
Axon-like protrusions promote small cell lung cancer migration and metastasis
Yang D, Qu F, Cai H, Chuang C, Lim J, Jahchan N, Grüner B, S Kuo C, Kong C, Oudin M, Winslow M, Sage J
eLife 2019;8
eLife 2019;8
Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells
Strange D, Jiyarom B, Pourhabibi Zarandi N, Xie X, Baker C, Sadri-Ardekani H, Shi P, Verma S, Lipkin W
mBio 2019;10(4)
mBio 2019;10(4)
Crebbp Loss Drives Small Cell Lung Cancer and Increases Sensitivity to HDAC Inhibition
Jia D, Augert A, Kim D, Eastwood E, Wu N, Ibrahim A, Kim K, Dunn C, Pillai S, Gazdar A, Bolouri H, Park K, MacPherson D
Cancer Discovery 2018;8(11):1422-1437
Cancer Discovery 2018;8(11):1422-1437
Intertumoral Heterogeneity in SCLC Is Influenced by the Cell Type of Origin
Yang D, Denny S, Greenside P, Chaikovsky A, Brady J, Ouadah Y, Granja J, Jahchan N, Lim J, Kwok S, Kong C, Berghoff A, Schmitt A, Reinhardt H, Park K, Preusser M, Kundaje A, Greenleaf W, Sage J, Winslow M
Cancer Discovery 2018;8(10):1316-1331
Cancer Discovery 2018;8(10):1316-1331
UCHL1-HIF-1 axis-mediated antioxidant property of cancer cells as a therapeutic target for radiosensitization
Nakashima R, Goto Y, Koyasu S, Kobayashi M, Morinibu A, Yoshimura M, Hiraoka M, Hammond E, Harada H
Scientific Reports 2017;7(1)
Scientific Reports 2017;7(1)
Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer
Lim J, Ibaseta A, Fischer M, Cancilla B, O’Young G, Cristea S, Luca V, Yang D, Jahchan N, Hamard C, Antoine M, Wislez M, Kong C, Cain J, Liu Y, Kapoun A, Garcia K, Hoey T, Murriel C, Sage J
Nature 2017;545(7654):360-364
Nature 2017;545(7654):360-364
Genetic requirement forMycland efficacy of RNA Pol I inhibition in mouse models of small cell lung cancer
Kim D, Wu N, Kim Y, Cheng P, Basom R, Kim D, Dunn C, Lee A, Kim K, Lee C, Singh A, Gazdar A, Harris C, Eisenman R, Park K, MacPherson D
Genes & Development 2016;30(11):1289-1299
Genes & Development 2016;30(11):1289-1299
The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin
Hidaka T, Ogawa E, Kobayashi E, Suzuki T, Funayama R, Nagashima T, Fujimura T, Aiba S, Nakayama K, Okuyama R, Yamamoto M
Nature Immunology 2016;18(1):64-73
Nature Immunology 2016;18(1):64-73
The prognostic potential and oncogenic effects of PRR11 expression in hilar cholangiocarcinoma
Chen Y, Cha Z, Fang W, Qian B, Yu W, Li W, Yu G, Gao Y
Oncotarget 2015;6(24):20419-20433
Oncotarget 2015;6(24):20419-20433
Antibody-based proteomics for discovery and exploration of proteins expressed in pancreatic islets.
Lindskog C, Asplund A, Engkvist M, Uhlen M, Korsgren O, Ponten F
Discovery medicine 2010 Jun;9(49):565-78
Discovery medicine 2010 Jun;9(49):565-78
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA005993 antibody. Corresponding UCHL1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
