Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB110-58874 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-58874, RRID:AB_877618
- Product name
- Rabbit Polyclonal UCH-L1/PGP9.5 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Aging of mouse intervertebral disc and association with back pain.
Peroneal tendoscopy.
Vincent K, Mohanty S, Pinelli R, Bonavita R, Pricop P, Albert TJ, Dahia CL
Bone 2019 Jun;123:246-259
Bone 2019 Jun;123:246-259
Peroneal tendoscopy.
Marmotti A, Cravino M, Germano M, Del Din R, Rossi R, Tron A, Tellini A, Castoldi F
Current reviews in musculoskeletal medicine 2012 Jun;5(2):135-44
Current reviews in musculoskeletal medicine 2012 Jun;5(2):135-44
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Blot of whole bovine brain extract stained with RPCA-UCHL1 showing a strong and clean band at about 24kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Simple Western lane view shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of different tissue and cell lysates using rabbit pAb to UCHL1, NB110-58874, dilution 1:2,000 in red, and mouse mAb to HSP60, dilution 1:10,000, in green: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24kDa corresponds to the UCHL1 protein, while the 60kDa band represents HSP60 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties but not in HeLa, NIH-3T3 and other non-neuronal cells.
