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- References [19]
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- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 13659-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13659-1-AP, RRID:AB_2107937
- Product name
- GART antibody
- Antibody type
- Polyclonal
- Description
- GART antibody (Cat. #13659-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues.
Nutrient requirements of organ-specific metastasis in breast cancer.
De novo purine synthesis reprograms the macrophage inflammatory response and the immune response in sepsis.
LEDGF Binds H3R17me2a Promoting De Novo Nucleotide Biosynthesis in SETD2 Mutant Clear Cell Renal Cell Carcinoma.
Oxyberberine alleviates fructose-induced hyperuricemia by modulating purine metabolism and gut microbiota.
Mechanistic insights into metformin's anti-hyperuricemic effect: Targeting PPP/DNPB/XOD-mediated purine pathway, purinosome assembly, and gut microbiota homostasis in rats.
The protection of UCK2 protein stability by GART maintains pyrimidine salvage synthesis for HCC growth under glucose limitation.
Succinate dehydrogenase activity supports de novo purine synthesis.
GART promotes multiple myeloma malignancy via tumor stemness mediated by activating the HSP90α/CDK6/β-catenin axis.
Prolonged Fructose Overconsumption Activates Original Biosynthetic and Metabolic Pathways of Endogenous Purine in Rats.
Site of breast cancer metastasis is independent of single nutrient levels.
De novo and salvage purine synthesis pathways across tissues and tumors.
GART Functions as a Novel Methyltransferase in the RUVBL1/β-Catenin Signaling Pathway to Promote Tumor Stemness in Colorectal Cancer.
Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.
Mitochondrial NADP(+) is essential for proline biosynthesis during cell growth.
UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.
ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.
Purine Nucleotide Availability Regulates mTORC1 Activity through the Rheb GTPase.
mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.
Kourtis S, Gañez Zapater A, Elbæk CR, Schmidt A, Ghose R, Coll Manzano A, Espinar Calvo L, Guirola M, Pardo-Lorente N, Garcia-Cao M, Pfeiffer M, Haynes S, Fontaine F, Müller A, Sdelci S
Nature communications 2026 Mar 6;17(1)
Nature communications 2026 Mar 6;17(1)
Nutrient requirements of organ-specific metastasis in breast cancer.
Abbott KL, Subudhi S, Ferreira R, Gültekin Y, Steinbuch SC, Munim MB, Ramesh DL, Honeder SE, Kumar AS, Wu M, Hansen JA, Shevzov-Zebrun A, Rashan EH, Eghbalian KM, Sivanand S, Barbeau AM, Riedmayr LM, Duquette M, Ali A, Henning N, Trojan SE, Waite M, Kunchok T, Nakano MA, Gourgue F, Ferraro GB, Do BT, Spanoudaki V, Sánchez-Rivera FJ, Jin X, Church GM, Jain RK, Vander Heiden MG
Nature 2026 Jan;649(8099):1292-1301
Nature 2026 Jan;649(8099):1292-1301
De novo purine synthesis reprograms the macrophage inflammatory response and the immune response in sepsis.
Haskó G, Liu L, Németh ZH, Wagener G, Akcan U, Arif M, Pacher P, Kelestemur T
Research square 2026 Feb 19;
Research square 2026 Feb 19;
LEDGF Binds H3R17me2a Promoting De Novo Nucleotide Biosynthesis in SETD2 Mutant Clear Cell Renal Cell Carcinoma.
Zhang Y, Zhou Y, Zhang Y, Lv J, Shen Y, Zhang D, Liu B, Zhao W, Ju J, Zhu Q, Wang K, Feng N
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(35):e16809
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(35):e16809
Oxyberberine alleviates fructose-induced hyperuricemia by modulating purine metabolism and gut microbiota.
Tan W, Xie X, Yu Q, Yan G, Chen H, Xie Q, Liu Y, Huang X, Chen J, Xie J, Su Z, Li Y
European journal of pharmacology 2025 Oct 15;1005:178061
European journal of pharmacology 2025 Oct 15;1005:178061
Mechanistic insights into metformin's anti-hyperuricemic effect: Targeting PPP/DNPB/XOD-mediated purine pathway, purinosome assembly, and gut microbiota homostasis in rats.
Yu Q, Liao H, Tan W, Xu L, Lin G, Ai Z, Xie Q, Liu Y, Li Y, Yang X, Su Z, Ma G, Xie J, Lai Z
Chemico-biological interactions 2025 Nov 1;421:111783
Chemico-biological interactions 2025 Nov 1;421:111783
The protection of UCK2 protein stability by GART maintains pyrimidine salvage synthesis for HCC growth under glucose limitation.
Sha N, Zhou B, Hou G, Xi Z, Wang W, Yan M, He J, Zhou Y, Xia Q, Jiang Y, Zhao Q
Oncogene 2025 May;44(16):1078-1092
Oncogene 2025 May;44(16):1078-1092
Succinate dehydrogenase activity supports de novo purine synthesis.
Nengroo MA, Klein AT, Carr HS, Vidal-Cruchez O, Sahu U, McGrail DJ, Sahni N, Gao P, Asara JM, Shah H, Mendillo ML, Ben-Sahra I
bioRxiv : the preprint server for biology 2025 Mar 1;
bioRxiv : the preprint server for biology 2025 Mar 1;
GART promotes multiple myeloma malignancy via tumor stemness mediated by activating the HSP90α/CDK6/β-catenin axis.
Qian J, Meng H, Wang Z, Sun Y, Xu X, Shi H, Wang C, Zhou L, Lv X, Yang Y, Gu C
European journal of pharmacology 2025 Jun 5;996:177584
European journal of pharmacology 2025 Jun 5;996:177584
Prolonged Fructose Overconsumption Activates Original Biosynthetic and Metabolic Pathways of Endogenous Purine in Rats.
Yu Q, Tan W, Ma X, Xu L, Lin G, Cheng J, Xie Q, Liu Y, Li Y, Xian Y, Lin Z, Chen J, Su Z, Yang X, Xie J
Molecular nutrition & food research 2025 Aug;69(16):e70122
Molecular nutrition & food research 2025 Aug;69(16):e70122
Site of breast cancer metastasis is independent of single nutrient levels.
Abbott KL, Subudhi S, Ferreira R, Gültekin Y, Steinbuch SC, Munim MB, Honeder SE, Kumar AS, Ramesh DL, Wu M, Hansen JA, Sivanand S, Riedmayr LM, Duquette M, Ali A, Henning N, Shevzov-Zebrun A, Gourgue F, Barbeau AM, Waite M, Kunchok T, Ferraro GB, Do BT, Spanoudaki V, Sánchez-Rivera FJ, Jin X, Church GM, Jain RK, Vander Heiden MG
bioRxiv : the preprint server for biology 2024 Oct 25;
bioRxiv : the preprint server for biology 2024 Oct 25;
De novo and salvage purine synthesis pathways across tissues and tumors.
Tran DH, Kim D, Kesavan R, Brown H, Dey T, Soflaee MH, Vu HS, Tasdogan A, Guo J, Bezwada D, Al Saad H, Cai F, Solmonson A, Rion H, Chabatya R, Merchant S, Manales NJ, Tcheuyap VT, Mulkey M, Mathews TP, Brugarolas J, Morrison SJ, Zhu H, DeBerardinis RJ, Hoxhaj G
Cell 2024 Jul 11;187(14):3602-3618.e20
Cell 2024 Jul 11;187(14):3602-3618.e20
GART Functions as a Novel Methyltransferase in the RUVBL1/β-Catenin Signaling Pathway to Promote Tumor Stemness in Colorectal Cancer.
Tang C, Ke M, Yu X, Sun S, Luo X, Liu X, Zhou Y, Wang Z, Cui X, Gu C, Yang Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Sep;10(25):e2301264
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Sep;10(25):e2301264
Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.
Soflaee MH, Kesavan R, Sahu U, Tasdogan A, Villa E, Djabari Z, Cai F, Tran DH, Vu HS, Ali ES, Rion H, O'Hara BP, Kelekar S, Hallett JH, Martin M, Mathews TP, Gao P, Asara JM, Manning BD, Ben-Sahra I, Hoxhaj G
Nature communications 2022 May 16;13(1):2698
Nature communications 2022 May 16;13(1):2698
Mitochondrial NADP(+) is essential for proline biosynthesis during cell growth.
Tran DH, Kesavan R, Rion H, Soflaee MH, Solmonson A, Bezwada D, Vu HS, Cai F, Phillips JA 3rd, DeBerardinis RJ, Hoxhaj G
Nature metabolism 2021 Apr;3(4):571-585
Nature metabolism 2021 Apr;3(4):571-585
UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.
Feng X, Ma D, Zhao J, Song Y, Zhu Y, Zhou Q, Ma F, Liu X, Zhong M, Liu Y, Xiong Y, Qiu X, Zhang Z, Zhang H, Zhao Y, Zhang K, Hong X, Zhang Z
The EMBO journal 2020 Mar 2;39(5):e102541
The EMBO journal 2020 Mar 2;39(5):e102541
ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.
Ali ES, Sahu U, Villa E, O'Hara BP, Gao P, Beaudet C, Wood AW, Asara JM, Ben-Sahra I
Molecular cell 2020 Jun 18;78(6):1178-1191.e6
Molecular cell 2020 Jun 18;78(6):1178-1191.e6
Purine Nucleotide Availability Regulates mTORC1 Activity through the Rheb GTPase.
Emmanuel N, Ragunathan S, Shan Q, Wang F, Giannakou A, Huser N, Jin G, Myers J, Abraham RT, Unsal-Kacmaz K
Cell reports 2017 Jun 27;19(13):2665-2680
Cell reports 2017 Jun 27;19(13):2665-2680
mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.
Ben-Sahra I, Hoxhaj G, Ricoult SJH, Asara JM, Manning BD
Science (New York, N.Y.) 2016 Feb 12;351(6274):728-733
Science (New York, N.Y.) 2016 Feb 12;351(6274):728-733
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 13659-1-AP(GART antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human cervical cancer using 13659-1-AP(GART antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The GART antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GART. This antibody recognizes human, mouse, rat antigen. The GART antibody has been validated for the following applications: ELISA, WB, IHC analysis.
