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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00002618-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002618-M01, RRID:AB_539058
- Product name
- Mouse Monoclonal GART Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GART - phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Hypoxia drives the assembly of the multienzyme purinosome complex.
Proteomic and Bioinformatic Studies for the Characterization of Response to Pemetrexed in Platinum Drug Resistant Ovarian Cancer.
Detecting Purinosome Metabolon Formation with Fluorescence Microscopy.
CRISPR-Cas9 induced mutations along de novo purine synthesis in HeLa cells result in accumulation of individual enzyme substrates and affect purinosome formation.
Mass spectrometric/bioinformatic identification of a protein subset that characterizes the cellular activity of anticancer peptides.
Transiently transfected purine biosynthetic enzymes form stress bodies.
No Evidence for Mutations that Deregulate GARS-AIRS-GART Protein Levels in Children with Down Syndrome.
Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease.
5,10-Methenyltetrahydrofolate synthetase activity is increased in tumors and modifies the efficacy of antipurine LY309887.
Doigneaux C, Pedley AM, Mistry IN, Papayova M, Benkovic SJ, Tavassoli A
The Journal of biological chemistry 2020 Jul 10;295(28):9551-9566
The Journal of biological chemistry 2020 Jul 10;295(28):9551-9566
Proteomic and Bioinformatic Studies for the Characterization of Response to Pemetrexed in Platinum Drug Resistant Ovarian Cancer.
Severi L, Losi L, Fonda S, Taddia L, Gozzi G, Marverti G, Magni F, Chinello C, Stella M, Sheouli J, Braicu EI, Genovese F, Lauriola A, Marraccini C, Gualandi A, D'Arca D, Ferrari S, Costi MP
Frontiers in pharmacology 2018;9:454
Frontiers in pharmacology 2018;9:454
Detecting Purinosome Metabolon Formation with Fluorescence Microscopy.
Pedley AM, Benkovic SJ
Methods in molecular biology (Clifton, N.J.) 2018;1764:279-289
Methods in molecular biology (Clifton, N.J.) 2018;1764:279-289
CRISPR-Cas9 induced mutations along de novo purine synthesis in HeLa cells result in accumulation of individual enzyme substrates and affect purinosome formation.
Baresova V, Krijt M, Skopova V, Souckova O, Kmoch S, Zikanova M
Molecular genetics and metabolism 2016 Nov;119(3):270-277
Molecular genetics and metabolism 2016 Nov;119(3):270-277
Mass spectrometric/bioinformatic identification of a protein subset that characterizes the cellular activity of anticancer peptides.
Genovese F, Gualandi A, Taddia L, Marverti G, Pirondi S, Marraccini C, Perco P, Pelà M, Guerrini R, Amoroso MR, Esposito F, Martello A, Ponterini G, D'Arca D, Costi MP
Journal of proteome research 2014 Nov 7;13(11):5250-61
Journal of proteome research 2014 Nov 7;13(11):5250-61
Transiently transfected purine biosynthetic enzymes form stress bodies.
Zhao A, Tsechansky M, Swaminathan J, Cook L, Ellington AD, Marcotte EM
PloS one 2013;8(2):e56203
PloS one 2013;8(2):e56203
No Evidence for Mutations that Deregulate GARS-AIRS-GART Protein Levels in Children with Down Syndrome.
Banerjee D, Ghosh D, Chatterjee A, Sinha S, Nandagopal K
Indian journal of clinical biochemistry : IJCB 2012 Jan;27(1):46-51
Indian journal of clinical biochemistry : IJCB 2012 Jan;27(1):46-51
Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease.
Noelker C, Schwake M, Balzer-Geldsetzer M, Bacher M, Popp J, Schlegel J, Eggert K, Oertel WH, Klockgether T, Dodel RC
Neuroscience letters 2012 Jan 17;507(1):10-5
Neuroscience letters 2012 Jan 17;507(1):10-5
5,10-Methenyltetrahydrofolate synthetase activity is increased in tumors and modifies the efficacy of antipurine LY309887.
Field MS, Anguera MC, Page R, Stover PJ
Archives of biochemistry and biophysics 2009 Jan 15;481(2):145-50
Archives of biochemistry and biophysics 2009 Jan 15;481(2):145-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of GART expression in transfected 293T cell line by GART monoclonal antibody (M01), clone 4D6-1D5.Lane 1: GART transfected lysate(46 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] - GART monoclonal antibody (M01), clone 4D6-1D5 Analysis of GART expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of monoclonal antibody to GART on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of GART transfected lysate using anti-GART monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GART MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of monoclonal antibody to GART on formalin-fixed paraffin-embedded human endometrium tissue. Antibody concentration 2 ug/ml.
