TA345794

antibody from OriGene

Targeting: ACO1 IREB1, IREBP, IRP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA345794

Provider

OriGene

Product name

Rabbit Polyclonal Anti-ACO1 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal Anti-ACO1 Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

ACO1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Lanes: 1. 60 ug mouse liver extract 2. 60 ug mouse liver extract 3. 60 ug mouse liver extract; Primary Antibody Dilution: 1: 500; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1: 3000; Gene Name: ACO1;

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Lanes: 1. 45ug capan1 cell lysate; 2. 45 ug HPAF cell lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-Rabbit HRP; Secondary Antibody Dilution: 1: 5000; Gene Name: ACO1;

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Lanes: Lane 1: 100ug HePG2 lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Goat anti rabbit-HRP; Secondary Antibody Dilution: 1: 8000; Gene Name: ACO1;

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB Suggested Anti-ACO1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human kidney

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Sample Type: Human Capan1 cells (Pancreatic cancer cell line) Primary Antibody Dilution: 1: 300; Secondary Antibody: Anti-rabbit-AlexaFluor-488; Secondary Antibody: ilution: 1: 200; Color/Signal Descriptions: ACO1: Green DAPI: Blue; Gene Name: ACO1;

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Rabbit Anti-ACO1 Antibody; Paraffin Embedded Tissue: Human Muscle; Cellular Data: Skeletal muscle cells; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X

Validation comment

IHC