H00000048-M01

antibody from Abnova Corporation

Targeting: ACO1 IREB1, IREBP, IRP1

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000048-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000048-M01, RRID:AB_464260

Product name

ACO1 monoclonal antibody (M01), clone 2C1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ACO1.

Antigen sequence

RDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVI
PLEYLPGENADALGLTGQERYTIIIPENLKPQMKV
QVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMI
RKMAK


Isotype

IgG

Antibody clone number

2C1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

IF/TA-related metabolic changes--proteome analysis of rat renal allografts.

Reuter S, Reiermann S, Wörner R, Schröter R, Edemir B, Buck F, Henning S, Peter-Katalinic J, Vollenbröker B, Amann K, Pavenstädt H, Schlatter E, Gabriëls G
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Aug;25(8):2492-501

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ACO1 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of ACO1 transfected lysate using anti-ACO1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ACO1 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol