NBP1-87677

antibody from Novus Biologicals

Targeting: ACO1 IREB1, IREBP, IRP1

Immunocytochemistry

Antibody data

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Validation data

Product number: NBP1-87677 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-87677, RRID:AB_11024630
Product name: Rabbit Polyclonal Aconitase 1 Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified. Specificity of human Aconitase 1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

ACO1 protein structure - NBP1-87677 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Aconitase 1 Antibody [NBP1-87677] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Aconitase 1 Antibody [NBP1-87677] - Analysis in human cell line U-87 MG.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Aconitase 1 Antibody [NBP1-87677] - Analysis in human cell lines U2OS and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Aconitase 1 Antibody [NBP1-87677] - Effect of HFD on IRP1. Densitometric analysis of IRP1 protein levels; beta-actin is shown as the loading control. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0116855), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Aconitase 1 Antibody [NBP1-87677] - Effect of fatty acids on IRP1 expression in HepG2 hepatocytes. IRP1 protein levels were evaluated by Western Blotting. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0116855), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] - Staining of human kidney shows high expression.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] - Staining of human lymph node shows low expression as expected.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] - Staining in human kidney and tonsil tissues.. Corresponding ACO1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.