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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-27824 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aconitase 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116.
- Concentration
- 0.78 mg/mL
Submitted references Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism.
Rychtarcikova Z, Lettlova S, Tomkova V, Korenkova V, Langerova L, Simonova E, Zjablovskaja P, Alberich-Jorda M, Neuzil J, Truksa J
Oncotarget 2017 Jan 24;8(4):6376-6398
Oncotarget 2017 Jan 24;8(4):6376-6398
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Aconitase 1 Polyclonal Antibody detects Aconitase 1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract. C. 30 µg HepG2 whole cell lysate/extract.7.5 % SDS-PAGE. Aconitase 1 Polyclonal Antibody (Product # PA5-27824) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Aconitase 1 was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: Aconitase 1 Polyclonal Antibody (Product # PA5 27824) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
