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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- AM1846b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1846b, RRID:AB_10819249
- Product name
- ALDH1A1 Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Purified Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.32 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- ALDH1A1 Monoclonal Antibody(Cat. #AM1846b) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the ALDH1A1 antibody detected the ALDH1A1 protein (arrow).
- Primary Ab dilution
- 1:500~1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1846b ALDH1A1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells. "
- Primary Ab dilution
- 1:100
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ALDH1A1 Antibody (Cat#AM1846b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- ALDH1A1 Monoclonal Antibody (Cat. #AM1846b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100
