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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-34623 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH1A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A549.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ALDH1A1 was performed by separating 30 µg of A549 lysates by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a ALDH1A1 Polyclonal Antibody (Product # PA5-34623) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ALDH1A1 was performed by separating 30 µg of A549 lysates by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a ALDH1A1 Polyclonal Antibody (Product # PA5-34623) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ALDH1A1 was performed by separating 50 µg of mouse liver lysates/extracts by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a ALDH1A1 Polyclonal Antibody (Product # PA5-34623) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ALDH1A1 Polyclonal Antibody(Product # PA5-34623) and a 55kDa band corresponding to ALDH1A1 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), SK-O-V3 (Lane 4), NIH:OVCAR-3 (Lane 5), Mouse Liver (Lane 6), Mouse Mammary gland (Lane 7), Rat Liver (Lane 8), Rat Mammary gland (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Expression of ALDH1A1 was found to be higher in A549,Hep G2 and NIH:OVCAR-3, Mouse liver and rat liver as compared to MCF-7,SK-O-V3, Mouse mammary gland and rat mammary gland.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of methanol-fixed HeLa cells using anti-ALDH1A1 Polyclonal Antibody (Product # PA5-34623) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ALDH1A1 in methanol-fixed HeLa cells using ALDH1A1 Polyclonal Antibody (Product # PA5-34623) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ALDH1A1 was performed in paraffin-embedded mouse liver tissue using ALDH1A1 Polyclonal Antibody (Product # PA5-34623) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ALDH1A1 was performed in paraffin-embedded gastric N87 xenograft tissue using ALDH1A1 Polyclonal Antibody (Product # PA5-34623) at a dilution of 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
