Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-7595 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-ALDHIA1 Antibody
- Antibody type
- Monoclonal
- Description
- Aldehyde dehydrogenase 1A1 (ALDH1A1) is a member of a superfamily of detoxification enzymes found in various tissues that participate in the oxidation of both aliphatic and aromatic aldehydes. The human ALDH1A1, which is tetrameric and predominantly of cytosolic origin, functions mainly in acetaldehyde and neurotransmitter metabolism. It is also reported to play a major role in the production of retinoic acid, which is important for gene expression and tissue differentiation, and also in cyclophosphamide detoxification. ALDH1A1 is found in various tissues, including the central nervous system (CNS), with highest levels in the liver. In the brain, ALDH1A1 participates in the metabolism of catecholamines including dopamine (DA) and norepinephrine, and is uniquely expressed in a subset of dopaminergic (DAergic) neurons in the ventral mesencephalon where it converts 3,4-dihydroxyphenylacetaldehyde, a potentially toxic aldehyde, to 3,4-dihydroxyphenylacetic acid, a non toxic metabolite. ALDHA1 has been implicated in the development of alcohol dependence and other alcohol-use disorders, alcohol-induced flushing and sensitivity to alcohol.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant protei
n of ALDHIA1 (amino acid 300-500) w
as used as the immunogen for this a
ntibody.- Isotype
- IgG
- Antibody clone number
- ABM5F21
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Expression analysis of ALDHIA1. Anti- ALDHIA1 antibody (Clone: ABM5F21) was tested at 2 µg/ml on (1) A549, (2) h Testis, (3) h Liver and (4) h Intestine lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flow analysis of ALDHIA1 on HepG2 cells using 0.5 µg/10^6 cells of ALDHIA1 antibody (Clone: ABM5F21). Green represents isotype control; red represents anti-ALDHIA1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
- Protocol
- Protocol