Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-11537 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH1A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and non-human primate based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH1A1 (arrow) using an ALDH1A1 polyclonal antibody (Product # PA5-11537) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A-431 (Lane 2), MCF7 (Lane 3), PC-3 (Lane 4), MDA-MB-231 (Lane 5) and tissue extract of Mouse Liver (Lane 6). The blot was probed with Anti-ALDH1A1 Polyclonal Antibody (Product # PA5-11537, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 55 kDa band corresponding to ALDH1A1 was detected across the cell lines tested except for A-431 and MCF7 which are reported negative for ALDH1A1 expression.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis using an ALDH1A1 polyclonal antibody (Product # PA5-11537) in HeLa cells. 0.025 mg/mL primary antibody was followed by fluor dye-conjugated donkey anti-rabbit lgG (H+L) (orange). Blue counterstaining is DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using an ALDH1A1 polyclonal antibody (Product # PA5-11537), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HepG2 cells using an ALDH1A1 polyclonal antibody (Product # PA5-11537) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody