Ab184310

antibody from Aladdin Scientific

Targeting: ALDH1A1 ALDH1, PUMB1, RALDH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab184310

Provider

Aladdin Scientific

Product name

ALDH1A1 Mouse mAb

Antibody type

Monoclonal

Description

WB: 1/1,000 - 1/20,000; Predicted molecular weight: 54.9 KDaICC/IF: 1/200

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

AB03/3F8

Vial size

100_l,10_l,1ml,50_l

Concentration

1 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C or -80¡C. Avoid freeze / thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

ALDH1A1 Mouse mAb (Ab184310) - Western Blot All lanes: ALDH1A1 Mouse Antibody (Ab184310) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 53 kDa Exposure time: 7.6 s 

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

ALDH1A1 Mouse mAb (Ab184310) - ICC/IF IF analysis of ALDH1A1 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with ALDH1A1 Mouse mAb (Ab184310) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.