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Immunohistochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB250-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal LIF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human LIF in ELISAs and Western blots. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha , rhIL-1 beta , rhIL-2, rhIL-3, rhIL-4, rhIL-6, rhIL-7, rhIL-8, rhG-CSF, rhGM-CSF, rhOSM, rhTGF-beta 1, rhTNF-alpha , rhTNF-beta , recombinant mouse (rm) IL-1 beta , rmIL-3, rmIL-4, rmIL-5, rmIL-6, rmIL-7, rmGM-CSF, bovine (b) FGF acidic, bFGF basic, human (h) PDGF, porcine (p) PDGF, hTGF-beta 1, pTGF-beta 1.2, or pTGF-beta 2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 1.5 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB250). The ND50 is typically 0.06-0.2 µg/mL.
